DNA fragments on a single-molecule degree reveals patterns of degradation in historic

convertex-chemie

BCAS2, a protein enriched in superior prostate most cancers, interacts with NBS1 to reinforce DNA double-strand break restore

Background: Breast most cancers amplified sequence 2 (BCAS2) performs essential roles in pre-mRNA splicing and androgen receptor transcription. Earlier research urged that BCAS2 is concerned in double-strand breaks (DSB); due to this fact, we aimed to characterise its mechanism and position in prostate most cancers (PCa).

Strategies: Western blotting and immunofluorescence microscopy have been used to assay the roles of BCAS2 within the DSBs of PCa cells and apoptosis in Drosophila, respectively. The impact of BCAS2 dosage on non-homologous finish becoming a member of (NHEJ) and homologous recombination (HR) have been assayed by exact end-joining assay and move cytometry, respectively. Glutathione-S-transferase pulldown and co-immunoprecipitation assays have been used to find out whether or not and the way BCAS2 interacts with NBS1. The expression of BCAS2 and different proteins in human PCa was decided by immunohistochemistry.

Outcomes: BCAS2 helped restore radiation-induced DSBs effectively in each human PCa cells and Drosophila. BCAS2 enhanced each NHEJ and HR, probably by interacting with NBS1, which concerned the BCAS2 N-terminus as effectively as each the NBS1 N- and C-termini. The overexpression of BCAS2 was considerably related to larger Gleason and pathology grades and shorter survival in sufferers with PCa.

Conclusion: BCAS2 promotes two DSB restore pathways by interacting with NBS1, and it could have an effect on PCa development.

convertex-chemie
convertex-chemie

CSP Antibody

MBS8581566-01mLAF405S 0.1mL(AF405S)
EUR 465

CSP Antibody

MBS8581566-01mLAF610 0.1mL(AF610)
EUR 465

CSP Antibody

MBS8581566-01mLAF635 0.1mL(AF635)
EUR 465

CSP Antibody

MBS5315730-01mL 0.1mL
EUR 470

CSP Antibody

MBS5315730-5x01mL 5x0.1mL
EUR 1955

CSP Antibody

C15493-100ul 100μl
EUR 217
Description: CSP Rabbit Polyclonal Antibody

CSP Antibody

C15493-50ul 50μl
EUR 143.5
Description: CSP Rabbit Polyclonal Antibody

Polyclonal CSP Antibody

AMM05812G 0.1ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human CSP . This antibody is tested and proven to work in the following applications:

CSP-beta Antibody

abx432070-200ul 200 ul
EUR 343.2

CSP-beta Antibody

abx432070-100g 100 µg
EUR 250

Anti-CSP antibody

STJ92500 200 µl
EUR 236.4
Description: Rabbit polyclonal to CSP.

Anti-CSP Antibody

MBS820421-003mL 0.03mL
EUR 185

Anti-CSP Antibody

MBS820421-01mL 0.1mL
EUR 255

Anti-CSP Antibody

MBS820421-02mL 0.2mL
EUR 335

Anti-CSP Antibody

MBS820421-5x02mL 5x0.2mL
EUR 1420

Anti-CSP Antibody

MBS9239448-01mL 0.1mL
EUR 415

Anti-CSP Antibody

MBS9239448-5x01mL 5x0.1mL
EUR 1841

CSP Polyclonal Antibody

ABP51061-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of CSP from Human, Mouse, Rat. This CSP antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human CSP at AA range: 100-180

CSP Polyclonal Antibody

ABP51061-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of CSP from Human, Mouse, Rat. This CSP antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human CSP at AA range: 100-180

CSP Polyclonal Antibody

ABP51061-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of CSP from Human, Mouse, Rat. This CSP antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human CSP at AA range: 100-180

CSP Polyclonal Antibody

40789 100ul
EUR 319

CSP Polyclonal Antibody

40789-100ul 100ul
EUR 302.4

CSP Polyclonal Antibody

40789-50ul 50ul
EUR 224.4

CSP Polyclonal Antibody

E040789 100μg/100μl
EUR 255
Description: Available in various conjugation types.

CSP Polyclonal Antibody

E20-71135 100ug
EUR 225
Description: Available in various conjugation types.

CSP Polyclonal Antibody

E44H04427 100ul
EUR 255
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

CSP Polyclonal Antibody

BT-AP02274-100ul 100ul Ask for price
Description: DNAJC5 is a member of the J protein family. J proteins function in many cellular processes by regulating the ATPase activity of 70 kDa heat shock proteins. The encoded protein (DnaJ heat shock protein family (Hsp40) member C5) plays a role in membrane trafficking and protein folding, and has been shown to have anti-neurodegenerative properties. The encoded protein is known to play a role in cystic fibrosis and Huntington's disease. A pseudogene of DNAJC5 is located on the short arm of chromosome 8.

CSP Polyclonal Antibody

BT-AP02274-20ul 20ul Ask for price
Description: DNAJC5 is a member of the J protein family. J proteins function in many cellular processes by regulating the ATPase activity of 70 kDa heat shock proteins. The encoded protein (DnaJ heat shock protein family (Hsp40) member C5) plays a role in membrane trafficking and protein folding, and has been shown to have anti-neurodegenerative properties. The encoded protein is known to play a role in cystic fibrosis and Huntington's disease. A pseudogene of DNAJC5 is located on the short arm of chromosome 8.

CSP Polyclonal Antibody

BT-AP02274-50ul 50ul Ask for price
Description: DNAJC5 is a member of the J protein family. J proteins function in many cellular processes by regulating the ATPase activity of 70 kDa heat shock proteins. The encoded protein (DnaJ heat shock protein family (Hsp40) member C5) plays a role in membrane trafficking and protein folding, and has been shown to have anti-neurodegenerative properties. The encoded protein is known to play a role in cystic fibrosis and Huntington's disease. A pseudogene of DNAJC5 is located on the short arm of chromosome 8.

CSP Polyclonal Antibody

MBS9243248-01mL 0.1mL
EUR 415

CSP Polyclonal Antibody

MBS9243248-5x01mL 5x0.1mL
EUR 1841

CSP Polyclonal Antibody

MBS9407290-005mL 0.05mL
EUR 245

CSP Polyclonal Antibody

MBS9407290-01mL 0.1mL
EUR 305

CSP Polyclonal Antibody

MBS9407290-5x01mL 5x0.1mL
EUR 1230

CSP Polyclonal Antibody

MBS9233786-01mL 0.1mL
EUR 415

CSP Polyclonal Antibody

MBS9233786-5x01mL 5x0.1mL
EUR 1841

CSP Polyclonal Antibody

MBS8522116-01mg 0.1mg
EUR 305

CSP Polyclonal Antibody

MBS8522116-01mLAF405L 0.1mL(AF405L)
EUR 465

CSP Polyclonal Antibody

MBS8522116-01mLAF405S 0.1mL(AF405S)
EUR 465

CSP Polyclonal Antibody

MBS8522116-01mLAF610 0.1mL(AF610)
EUR 465

CSP Polyclonal Antibody

MBS8522116-01mLAF635 0.1mL(AF635)
EUR 465

CSP Polyclonal Antibody

MBS2535113-002mL 0.02mL
EUR 140

CSP Polyclonal Antibody

MBS2535113-006mL 0.06mL
EUR 180

CSP Polyclonal Antibody

MBS2535113-012mL 0.12mL
EUR 260

CSP Polyclonal Antibody

MBS2535113-02mL 0.2mL
EUR 405

CSP polyclonal antibody

MBS565353-01mg 0.1mg
EUR 495

CSP polyclonal antibody

MBS565353-5x01mg 5x0.1mg
EUR 2075

Plasmodium vivax CSP Antibody

abx022151-1mg 1 mg
EUR 944.4

Plasmodium vivax CSP Antibody

GWB-418779 1 mg Ask for price

Plasmodium Vivax CSP Antibody

GWB-92F840 0.2 mg Ask for price

Plasmodium vivax CSP Antibody

GWB-7738B9 1 mg Ask for price

Mouse Anti-P.falciparum CSP Recombinant Antibody

TRI-AB-012 0.1mg
EUR 1620

Mouse Anti Plasmodium Vivax Csp Monoclonal Antibody

DMABT-51495MP 0.2 mg
EUR 920.4

Mouse Anti-Plasmodium vivax CSP Monoclonal Antibody

DMAB4108 1 mg
EUR 1146

Rabbit anti-CSP Antibody

DL91793A-100ul 100 ul
EUR 325
Description: CSP; DnaJ homolog subfamily C member 5; Cysteine string protein; CSP

Rabbit anti-CSP Antibody

DL91793A-50ul 50 ul
EUR 227.5
Description: CSP; DnaJ homolog subfamily C member 5; Cysteine string protein; CSP

Rabbit anti-CSP Antibody

YLD2210-100ul 100 ul
EUR 320
Description: Rabbit polyclonal antibody to CSP

Rabbit anti-CSP Antibody

YLD2210-50ul 50 ul
EUR 200
Description: Rabbit polyclonal antibody to CSP

CSP Rabbit Polyclonal Antibody

ES2060-100ul 100ul
EUR 124
Description: A Rabbit Polyclonal antibody against CSP from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA

CSP Rabbit Polyclonal Antibody

ES2060-50ul 50ul
EUR 74
Description: A Rabbit Polyclonal antibody against CSP from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA

Rabbit anti-Caenorhabditis elegans csp-3 Polyclonal Antibody

MBS7147487-INQUIRE INQUIRE Ask for price

Rabbit anti-Caenorhabditis elegans csp-1 Polyclonal Antibody

MBS7143908-INQUIRE INQUIRE Ask for price

Rabbit anti-Caenorhabditis elegans csp-2 Polyclonal Antibody

MBS7170311-INQUIRE INQUIRE Ask for price

CSP Polyclonal Conjugated Antibody

C40789 100ul
EUR 476.4

CSP Polyclonal Conjugated Antibody

MBS9449718-01mLAF350 0.1mL(AF350)
EUR 480

CSP Polyclonal Conjugated Antibody

MBS9449718-01mLAF405 0.1mL(AF405)
EUR 480

CSP Polyclonal Conjugated Antibody

MBS9449718-01mLAF488 0.1mL(AF488)
EUR 480

CSP Polyclonal Conjugated Antibody

MBS9449718-01mLAF555 0.1mL(AF555)
EUR 480

CSP Polyclonal Conjugated Antibody

MBS9449718-01mLBiotin 0.1mL(Biotin)
EUR 480

Polyclonal DNAJC5 / CSP Antibody (aa177-191)

APR11768G 0.05mg
EUR 580.8
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human DNAJC5 / CSP (aa177-191). This antibody is tested and proven to work in the following applications:

Rabbit anti-Drosophila melanogaster (Fruit fly) Csp Polyclonal Antibody

MBS7150313-INQUIRE INQUIRE Ask for price

anti-CSP

YF-PA21083 50 ug
EUR 435.6
Description: Mouse polyclonal to CSP

Plasmodium vivax Circumsporozoite Protein (P. vivax CSP) Antibody

abx414618-02mg 0.2 mg
EUR 678

Anti-CSP-beta (mouse aa177-190) antibody

STJ73524 100 µg
EUR 312

CSP

5-01011 4 x 1mg Ask for price

CSP

MBS8246387-10mg 10mg
EUR 755

CSP

MBS8246387-1mg 1mg
EUR 270

CSP

MBS8246387-5mg 5mg
EUR 530

CSP

MBS8246387-5x10mg 5x10mg
EUR 3315

Goat anti-CSP-beta (mouse aa177-190) Antibody

dAP-3149 50ug
EUR 264.6

Goat anti-CSP-beta (mouse aa177-190) Antibody

MBS423397-01mg 0.1mg
EUR 275

Goat anti-CSP-beta (mouse aa177-190) Antibody

MBS423397-5x01mg 5x0.1mg
EUR 1095

CSP Peptide

abx266729-1ml 1 ml
EUR 412.5

CSP Peptide

abx266729-200l 200 µl
EUR 212.5

Plasmodium vivax (circumsporozoite (CSP) mouse monoclonal antibody, clone PVC-2

3PLV2-PVC-2 1 mg Ask for price

Plasmodium vivax CSP recombinant antigen

8968 100 ug
EUR 529.8
Description: This is Plasmodium vivax CSP recombinant antigen for ELISA,WB.

CSP-beta Peptide

43-589P 0.1 mg
EUR 405.6

Plasmodium vivax CSP protein, Recombinant (malaria CSP pv) Protein

GWB-T00016 1mg Ask for price

CSP Blocking Peptide

20-abx063987
  • Ask for price
  • Ask for price
  • 1 mg
  • 5 mg

CSP Blocking Peptide

MBS824034-1mg 1mg
EUR 190

CSP Blocking Peptide

MBS824034-5mg 5mg
EUR 345

CSP Blocking Peptide

MBS824034-5x5mg 5x5mg
EUR 1465

Mouse Monoclonal Anti-Circumsporozoite (CSP) (P. vivax) IgG, aff pure #1

CSPV11-M 100 ug
EUR 578.4

Mouse Monoclonal Anti-Circumsporozoite (CSP) (P. vivax) IgG, aff pure #2

CSPV12-M 100 ug
EUR 578.4

Cysteine String Protein (CSP)

MBS614840-01mg 0.1mg
EUR 685

Cysteine String Protein (CSP)

MBS614840-5x01mg 5x0.1mg
EUR 2935

Plasmodium vivax CSP Protein

abx060642-1mg 1 mg
EUR 3567.6

PLASMODIUM VIVAX CSP PROTEIN

GWB-D19F34 0.1 mg Ask for price

CSP-beta Immunizing Peptide

MBS428525-01mg 0.1mg
EUR 225

MAb to P. vivax CSP

MBS310454-INQUIRE INQUIRE Ask for price

anti- CSPP1 antibody

FNab02026 100µg
EUR 658.5
Description: Antibody raised against CSPP1

anti- CSPG4,NG2 antibody

FNab02025 100µg
EUR 606.3
Description: Antibody raised against CSPG4,NG2

Competence-Stimulating Peptide-2 (CSP-2)

T38559-10mg 10mg Ask for price
Description: Competence-Stimulating Peptide-2 (CSP-2)

Competence-Stimulating Peptide-2 (CSP-2)

T38559-1g 1g Ask for price
Description: Competence-Stimulating Peptide-2 (CSP-2)

Competence-Stimulating Peptide-2 (CSP-2)

T38559-1mg 1mg Ask for price
Description: Competence-Stimulating Peptide-2 (CSP-2)

Competence-Stimulating Peptide-2 (CSP-2)

T38559-50mg 50mg Ask for price
Description: Competence-Stimulating Peptide-2 (CSP-2)

Competence-Stimulating Peptide-2 (CSP-2)

T38559-5mg 5mg Ask for price
Description: Competence-Stimulating Peptide-2 (CSP-2)

Competence-Stimulating Peptide-2 (CSP-2)

MBS5799190-INQUIRE INQUIRE Ask for price

Anti-NG2/CSPG4 Antibody

MBS1753590-01mg 0.1mg
EUR 450

Anti-NG2/CSPG4 Antibody

MBS1753590-5x01mg 5x0.1mg
EUR 1870

Plasmodium vivax CSP Recombinant Protein

53-353 0.01 mg
EUR 714.3
Description: Plasmodium vivax CSP Recombinant Protein

RECOMBINANT PLASMODIUM VIVAX CSP PROTEIN

MBS232087-01mg 0.1mg
EUR 1145

RECOMBINANT PLASMODIUM VIVAX CSP PROTEIN

MBS232087-5x01mg 5x0.1mg
EUR 4980

Rabbit Anti-Circumsporozoite (CSP, P.falciparum) C-terminal (207-397 aa) His-tag protein antiserum

CSPF11-S 100 ul
EUR 548.4

Anti-Human CSPG5 Antibody

MBS1569935-01mg 0.1mg
EUR 405

Anti-Human CSPG5 Antibody

MBS1569935-5x01mg 5x0.1mg
EUR 1520

Bolle Cobra COBCSP CSP Lens - EACH

SAF1902 EACH
EUR 21.33

Recombinant (E. coli) Circumsporozoite (CSP; 353-aa and GST) antigen (P. vivax)

CSPV16-R 100 ug
EUR 634.8

CSP (DNAJC5) (NM_025219) Human Recombinant Protein

PROTQ9H3Z4 20ug
EUR 1249
Description: Recombinant protein of human DnaJ (Hsp40) homolog, subfamily C, member 5 (DNAJC5)

Recombinant Plasmodium Vivax CSP Protein [GST]

VAng-Wyb7856-100g 100 µg
EUR 1380
Description: Plasmodium Vivax CSP protein, recombinant protein.

OPMA04825-1MG - Plasmodium vivax CSP Protein

OPMA04825-1MG 1mg
EUR 2499

OPSA10797-100UG - PLASMODIUM VIVAX CSP Protein

OPSA10797-100UG 0.1mg
EUR 1139

YLKKIKNSL, P. falciparum circumsporozoite (CSP 334-342) polypeptide

CSPF15-P 1 mg
EUR 343.2

Anti-NG2/CSPG4 Antibody Picoband™

A03394-3 10ug
EUR 154
Description: Western blot, 0.25-0.5μg/ml, Human;_x000D_Direct ELISA, 0.1-0.5μg/ml, Human

Recombinant Plasmodium Vivax CSP (a.a. 353), GST-tagged

DAG448 1mg
EUR 2266.8

Anti-NG2/Cspg4 Antibody, Rabbit Polyclonal

MBS8100816-01mL 0.1mL
EUR 300

Anti-NG2/Cspg4 Antibody, Rabbit Polyclonal

MBS8100816-5x01mL 5x0.1mL
EUR 1200

Anti-NG2 Purified CSPG4 Monoclonal Antibody

M03394-1 0.1mg
EUR 291
Description: Boster Bio Anti-NG2 Purified CSPG4 Monoclonal Antibody (Catalog# M03394-1). Tested in Flow Cytometry, IP, WB, ICC application(s). This antibody reacts with Human.

CSP (DNAJC5) (NM_025219) Human Over-expression Lysate

LS080331 100ug
EUR 628
Description: Transient overexpression lysate of DnaJ (Hsp40) homolog, subfamily C, member 5 (DNAJC5)

Human Anti-Circumsporozoite protein (CSP, P falciparum/RTS,S vaccine) IgG ELISA kit, 96 tests, Quantitative

970-400-CHG 1 Kit
EUR 1000.8

Human Anti-Circumsporozoite protein (CSP, P falciparum/RTS,S vaccine) IgM ELISA kit, 96 tests, Quantitative

970-410-CHM 1 Kit
EUR 1000.8

Cysteine string protein (CSP) Alpha, DNAJC5 alpha

MBS395741-01mg 0.1mg
EUR 440

Cysteine string protein (CSP) Alpha, DNAJC5 alpha

MBS395741-5x01mg 5x0.1mg
EUR 1820

Cysteine string protein (CSP) Alpha, DNAJC5 alpha

MBS395909-01mg 0.1mg
EUR 425

Cysteine string protein (CSP) Alpha, DNAJC5 alpha

MBS395909-5x01mg 5x0.1mg
EUR 1760

Rabbit Anti-Circumsporozoite protein (CSP, P falciparum/RTS,S vaccine) IgG ELISA kit, 96 tests, Quantitative

970-200-CSR 1 Kit
EUR 1000.8

Rabbit Anti-Circumsporozoite protein (CSP, P falciparum/RTS,S vaccine) IgM ELISA kit, 96 tests, Quantitative

970-210-CSM 1 Kit
EUR 1000.8

Plasmodium vivax CSP Recombinant Protein GST liquid

IPMDPLAMDVVXCSPRGST10UG each
EUR 851
Description: Plasmodium vivax CSP Recombinant Protein GST liquid

Plasmodium vivax CSP Recombinant Protein GST liquid

MBS8434473-INQUIRE INQUIRE Ask for price

Recombinant Xenopus laevis Cysteine string protein (csp)

MBS1022748-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1115

Recombinant Xenopus laevis Cysteine string protein (csp)

MBS1022748-002mgEColi 0.02mg(E-Coli)
EUR 695

Recombinant Xenopus laevis Cysteine string protein (csp)

MBS1022748-002mgYeast 0.02mg(Yeast)
EUR 870

Recombinant Xenopus laevis Cysteine string protein (csp)

MBS1022748-01mgEColi 0.1mg(E-Coli)
EUR 835

Recombinant Xenopus laevis Cysteine string protein (csp)

MBS1022748-01mgYeast 0.1mg(Yeast)
EUR 1015

CSPG4/NG2 Rabbit anti-Human Polyclonal Antibody

MBS2403042-005mL 0.05mL
EUR 615

CSPG4/NG2 Rabbit anti-Human Polyclonal Antibody

MBS2403042-5x005mL 5x0.05mL
EUR 2605

CSPG4/NG2 Rabbit anti-Human Polyclonal Antibody

MBS2403108-005mL 0.05mL
EUR 615

CSPG4/NG2 Rabbit anti-Human Polyclonal Antibody

MBS2403108-5x005mL 5x0.05mL
EUR 2605

CSPG4/NG2 Rabbit anti-Human Polyclonal Antibody

MBS2403595-005mg 0.05mg
EUR 615

CSPG4/NG2 Rabbit anti-Human Polyclonal Antibody

MBS2403595-5x005mg 5x0.05mg
EUR 2605

Sequencing and Characterisation of Complete Mitochondrial DNA Genome for Trigonopoma pauciperforatum (Cypriniformes: Cyprinidae: Danioninae) with Phylogenetic Consideration

  • The Trigonopoma pauciperforatum or the redstripe rasbora is a cyprinid generally present in marshes and swampy areas with slight acidic tannin-stained water within the tropics. On this research, the whole mitogenome sequence of pauciperforatum was first amplified in two components utilizing two pairs of overlapping primers after which sequenced.
  • The dimensions of the mitogenome is 16,707 bp, encompassing 22 switch RNA genes, 13 protein-coding genes, two ribosomal RNA genes and a putative management area. An identical gene organisation was detected between this species and different members of the family.
  • The heavy strand accommodates 28 genes whereas the sunshine strand homes the remaining 9 genes. Most protein-coding genes utilise ATG as begin codon aside from COI gene which makes use of GTG as an alternative. The terminal related sequence (TAS), central conserved sequence block (CSB-F, CSB-D and CSB-E) in addition to variable sequence block (CSB-1, CSB-2 and CSB-3) are conserved within the management area.
  • The utmost probability phylogenetic tree revealed the divergence of pauciperforatum from the basal area of the foremost clade, the place its evolutionary relationships with Boraras maculatus, Rasbora cephalotaenia and R. daniconius are poorly resolved as urged by the low bootstrap values.
  • This work contributes in the direction of the genetic useful resource enrichment for peat swamp conservation and complete in-depth comparisons throughout different phylogenetic researches completed on the Rasbora-related genus.

Reconstructing double-stranded DNA fragments on a single-molecule degree reveals patterns of degradation in historic samples

Intensive manipulations concerned within the preparation of DNA samples for sequencing have hitherto made it unimaginable to find out the exact construction of double-stranded DNA fragments being sequenced, such because the presence of blunt ends, single-stranded overhangs, or single-strand breaks.

We right here describe MatchSeq, a technique that mixes single-stranded DNA library preparation from diluted DNA samples with computational sequence matching, permitting the reconstruction of double-stranded DNA fragments on a single-molecule degree.

 The appliance of MatchSeq to Neanderthal DNA, a very advanced supply of degraded DNA, reveals that 1- or 2-nt overhangs and blunt ends dominate the ends of historic DNA molecules and that brief gaps exist, that are predominantly brought on by the lack of particular person purines.

We additional present that deamination of cytosine to uracil happens in each single- and double-stranded contexts near the ends of molecules, and that single-stranded components of DNA fragments are enriched in pyrimidines. MatchSeq supplies unprecedented decision for interrogating the buildings of fragmented double-stranded DNA and could be utilized to fragmented double-stranded DNA remoted from any organic supply. The tactic depends on well-established laboratory methods and might simply be built-in into routine knowledge era.

This risk is proven by the profitable reconstruction of double-stranded DNA fragments from beforehand revealed single-stranded sequence knowledge, permitting a extra complete characterization of the biochemical properties not solely of historic DNA but in addition of cell-free DNA from human blood plasma, a clinically related marker for the analysis and monitoring of illness.

Orf Virus VEGF-E

MBS691947-0002mg 0.002mg
EUR 265

Orf Virus VEGF-E

MBS691947-5x0002mg 5x0.002mg
EUR 890

VEGF E (Orf Virus)

cyt-263 2µg
EUR 60
Description: Recombinant Vascular Endothelial Growth Factor-E (Orf Virus)

Orf Virus VEGF-E, Heparin-binding

MBS691480-002mg 0.02mg
EUR 440

Orf Virus VEGF-E, Heparin-binding

MBS691480-5x002mg 5x0.02mg
EUR 1685

Orf Virus VEGF-E, Heparin-binding

MBS691994-0005mg 0.005mg
EUR 300

Orf Virus VEGF-E, Heparin-binding

MBS691994-5x0005mg 5x0.005mg
EUR 1050

Orf virus VEGF-E Recombinant Protein

300-044 5 µg
EUR 136.5
Description: A DNA sequence encoding the mature variant of ov-VEGF-E isolate D1701 (Dehio et al., 1999; GenBank accession No. AF106020) was expressed in E. coli as a 132 amino acid residue fusion protein with an N-terminal His-tag sequence and a thrombin cleavage site. Recombinant VEGF-E homodimer was dimerized in vitro and has a predicted mass of approximately 35 kDa. Based on sequence similarity to VEGF-A, a gene encoding a VEGF homologue has recently been discovered in the genome of Orf virus (OV) (Lyttle et al., 1994). Different isolates of Orf virus show significant amino acid sequence similarity to VEGF-A and described as a viral virulence factor that appears to be derived from captured host genes. All eight Cysteine residues of the central Cysteine knot motif characteristic of members of the VEGF family are conserved among other residues in the VEGF-E proteins (Dehio et al., 1999; Wise et al., 1999). Alignment of all mammalian VEGF sequences indicated that VEGF-E is distinct from the previously described VEGFs but most closely related to VEGF-A. Like VEGF-A, VEGF-E was found to bind with high affinity to VEGF receptor-2 (KDR) resulting in receptor autophosphorylation, whilst in contrast to VEGF-A, VEGF-E cannot bind to VEGF receptor-1 (Flt-1). Furthermore VEGF-E can also not bind to VEGF receptor-3 (FLT-4). Therefore VEGF-E is a potent angiogenic factor selectively binding to VEGF receptor –2/KDR.

Orf virus VEGF-E Recombinant Protein

300-044S 2 µg
EUR 73.5
Description: A DNA sequence encoding the mature variant of ovVEGF-E isolate D1701 (Dehio et al., 1999; GenBank accession No. AF106020) was expressed in E. coli as a 132 amino acid residue fusion protein with an N-terminal His-tag sequence and a thrombin cleavage site. Recombinant VEGF-E homodimer was dimerized in vitro and has a predicted mass of approximately 35 kDa. Based on sequence similarity to VEGF-A, a gene encoding a VEGF homologue has recently been discovered in the genome of Orf virus (OV) (Lyttle et al., 1994). Different isolates of Orf virus show significant amino acid sequence similarity to VEGF-A and described as a viral virulence factor that appears to be derived from captured host genes. All eight cysteine residues of the central cysteine knot motif characteristic of members of the VEGF family are conserved among other residues in the VEGF-E proteins (Dehio et al., 1999; Wise et al., 1999). Alignment of all mammalian VEGF sequences indicated that VEGF-E is distinct from the previously described VEGFs but most closely related to VEGF-A. Like VEGF-A, VEGF-E was found to bind with high affinity to VEGF receptor-2 (KDR) resulting in receptor autophosphorylation, whilst in contrast to VEGF-A, VEGF-E can not bind to VEGF receptor-1 (Flt-1). Furthermore VEGF-E can also not bind to VEGF receptor-3 (FLT-4). Therefore VEGF-E is a potent angiogenic factor selectively binding to VEGF receptor –2/KDR.

Orf virus VEGF-E Recombinant Protein

300-045 20 µg
EUR 357
Description: A DNA sequence encoding the mature variant of ovVEGF-E isolate D1701 (Dehio et al., 1999; GenBank accession No. AF106020) was expressed in E. coli as a 132 amino acid residue fusion protein with an N-terminal His-tag sequence and a thrombin cleavage site. Recombinant VEGF-E homodimer was dimerized in vitro and has a predicted mass of approximately 35 kDa. Based on sequence similarity to VEGF-A, a gene encoding a VEGF homologue has recently been discovered in the genome of Orf virus (OV) (Lyttle et al., 1994). Different isolates of Orf virus show significant amino acid sequence similarity to VEGF-A and described as a viral virulence factor that appears to be derived from captured host genes. All eight cysteine residues of the central cysteine knot motif characteristic of members of the VEGF family are conserved among other residues in the VEGF-E proteins (Dehio et al., 1999; Wise et al., 1999). Alignment of all mammalian VEGF sequences indicated that VEGF-E is distinct from the previously described VEGFs but most closely related to VEGF-A. Like VEGF-A, VEGF-E was found to bind with high affinity to VEGF receptor-2 (KDR) resulting in receptor autophosphorylation, whilst in contrast to VEGF-A, VEGF-E can not bind to VEGF receptor-1 (Flt-1). Furthermore VEGF-E can also not bind to VEGF receptor-3 (FLT-4). Therefore VEGF-E is a potent angiogenic factor selectively binding to VEGF receptor –2/KDR.

Anti-Orf Virus VEGF-E Antibody

102-PA70 50 µg
EUR 115.5
Description: A DNA sequence encoding the mature variant of ov-VEGF-E isolate D1701 was expressed in E. coli as a 132 amino acid residue fusion protein with an N-terminal His-tag sequence and a thrombin cleavage site. Recombinant VEGF-E homodimer was dimerized in vitro and has a predicted mass of approximately 35 kDa. Based on sequence similarity to VEGF-A, a gene encoding a VEGF homologue has recently been discovered in the genome of Orf virus (OV). Different isolates of Orf virus show significant amino acid sequence similarity to VEGF-A and described as a viral virulence factor that appears to be derived from captured host genes. All eight Cysteine residues of the central Cysteine knot motif characteristic of members of the VEGF family are conserved among other residues in the VEGF-E proteins. Alignment of all mammalian VEGF sequences indicated that VEGF-E is distinct from the previously described VEGFs but most closely related to VEGF-A. Like VEGF-A, VEGF-E was found to bind with high affinity to VEGF receptor-2 (KDR) resulting in receptor autophosphorylation, whilst in contrast to VEGF-A, VEGF-E cannot bind to VEGF receptor-1 (Flt-1). Furthermore VEGF-E can also not bind to VEGF receptor-3 (FLT-4). Therefore VEGF-E is a potent angiogenic factor selectively binding to VEGF receptor –2/KDR.

Recombinant Virus VEGF E (Orf Virus) Protein, His, E.coli-1mg

QP10912-1mg 1mg
EUR 6181.2

Recombinant Virus VEGF E (Orf Virus) Protein, His, E.coli-2ug

QP10912-2ug 2ug
EUR 186

Recombinant Virus VEGF E (Orf Virus) Protein, His, E.coli-10ug

QP10912-10ug 10ug
EUR 241.2

Orf virus VEGF-E, Heparin-binding Recombinant Protein

300-046 20 µg
EUR 194.25
Description: A DNA sequence encoding the first 116 amino acid residue of Orf virus VEGF-E isolate D1701 (Dehio et al., 1999 EMBO J. 18:363-374; GenBank accession No. AF106020) was fused with a DNA sequence encoding to the C-terminal heparin binding domain of human VEGF165. The chimeric protein was expressed in insect cells using a baculovirus expression system. Based on sequence similarity to VEGF-A, a gene encoding a VEGF homologue has recently been discovered in the genome of Orf virus (OV) (Lyttle et al., 1994 J. Virol 68:84-92). Different isolates of orf virus show significant amino acid sequence similarity to VEGF-A and described as a viral virulence factor that appear to be derived from captured host genes. All eight cysteine residues of the central cysteine knot motif characteristic of members of the VEGF family are conserved among other residues in the VEGF-E proteins (Dehio et al., 1999 EMBO J. 18:363-374; Wise et al., 1999 Proc. Natl. Acad. Sci USA 96:3071-3076). Alignment of all mammalian VEGF sequences indicated that VEGF-E is distinct from the previously described VEGFs but most closely related to VEGF-A. Like VEGF-A, VEGF-E was found to bind with high affinity to VEGF receptor-2 (KDR) resulting in receptor autophosphorylation, whilst in contrast to VEGF-A, VEGF-E and hb-VEGF-E can not bind to VEGF receptor-1 (Flt-1). Therefore VEGF-E is a potent angiogenic factor selectively binding to VEGF receptor–2/ KDR. Compared to human VEGF165 this virus form has no heparin-binding domain and seems to be a freely secreted protein comparable to VEGF121. In order to compare this form with human VEGF165, an additional heparin-binding domain was engineered at the C-terminus to allow interaction with proteo-aminoglycans and heparan sulfate. These form is also able to interact with neuropillin–1.

Orf virus VEGF-E, Heparin-binding Recombinant Protein

300-046S 5 µg
EUR 94.5
Description: A DNA sequence encoding the first 116 amino acid residue of Orf virus VEGF-E isolate D1701 (Dehio et al., 1999 EMBO J. 18:363-374; GenBank accession No. AF106020) was fused with a DNA sequence encoding to the C-terminal heparin binding domain of human VEGF165. The chimeric protein was expressed in insect cells using a baculovirus expression system. Based on sequence similarity to VEGF-A, a gene encoding a VEGF homologue has recently been discovered in the genome of Orf virus (OV) (Lyttle et al., 1994 J. Virol 68:84-92). Different isolates of orf virus show significant amino acid sequence similarity to VEGF-A and described as a viral virulence factor that appear to be derived from captured host genes. All eight cysteine residues of the central cysteine knot motif characteristic of members of the VEGF family are conserved among other residues in the VEGF-E proteins (Dehio et al., 1999 EMBO J. 18:363-374; Wise et al., 1999 Proc. Natl. Acad. Sci USA 96:3071-3076). Alignment of all mammalian VEGF sequences indicated that VEGF-E is distinct from the previously described VEGFs but most closely related to VEGF-A. Like VEGF-A, VEGF-E was found to bind with high affinity to VEGF receptor-2 (KDR) resulting in receptor autophosphorylation, whilst in contrast to VEGF-A, VEGF-E and hb-VEGF-E can not bind to VEGF receptor-1 (Flt-1). Therefore VEGF-E is a potent angiogenic factor selectively binding to VEGF receptor–2/ KDR. Compared to human VEGF165 this virus form has no heparin-binding domain and seems to be a freely secreted protein comparable to VEGF121. In order to compare this form with human VEGF165, an additional heparin-binding domain was engineered at the C-terminus to allow interaction with proteo-aminoglycans and heparan sulfate. These form is also able to interact with neuropillin–1.

VEGF-E Vascular Endothelial Growth Factor-E Recombinant Protein(Orf Virus)

PROTQ2F842 Regular: 10ug
EUR 380.4
Description: A DNA sequence encoding the mature variant of ovVEGF-E isolate D1701 (Dehio et al., 1999; GenBank accession No. AF106020) was expressed in E. coli as a 132 amino acid residue fusion protein with an N-terminal His-tag sequence and a thrombin cleavage site. Recombinant VEGF-E homodimer was dimerized in vitro and has a predicted mass of approximately 35 kDa.

ov-VEGF-E (Orf virus) (Recombinant) Human

GWB-C8AD60 0.005 mg Ask for price

VEGFE protein (Orf virus)

30R-AV011 5 ug
EUR 381
Description: Purified recombinant VEGFE protein (Orf virus)

VEGFE protein (Orf virus)

MBS538323-0005mg 0.005mg
EUR 505

VEGFE protein (Orf virus)

MBS538323-5x0005mg 5x0.005mg
EUR 2125

Recombinant Orf Virus VEGFE Protein

VAng-Lsx0417-inquire inquire Ask for price
Description: Orf Virus VEGFE, recombinant protein from Baculovirus.

Recombinant Orf Virus VEGFE Protein [His]

VAng-Lsx0418-inquire inquire Ask for price
Description: Orf Virus VEGFE [His], recombinant protein from E. coli.

Deoxybrevianamide E

D083-1MG 1 mg
EUR 187.71
Description: C21H25N3O2

Deoxybrevianamide E

D083-5MG 5 mg
EUR 656.34
Description: C21H25N3O2

Berkeleylactone E

B087-1MG 1 mg
EUR 142.63
Description: C20H32O7  

Berkeleylactone E

B087-5MG 5 mg
EUR 498.97
Description: C20H32O7  

Chevalone E

C257-0.5MG 0.5 mg
EUR 150.43
Description: C20H24O4  

Chevalone E

C257-2.5MG 2.5 mg
EUR 525.85
Description: C20H24O4  

Cytochalasin E

C197-1MG 1 mg
EUR 77.6
Description: C28H33NO7

Cytochalasin E

C197-5MG 5 mg
EUR 270.51
Description: C28H33NO7

Oligomycin E

O021-1MG 1 mg
EUR 207.65
Description: C45H72O13

Oligomycin E

O021-5MG 5 mg
EUR 726.13
Description: C45H72O13

Paraherquamide E

P070-0.5MG 0.5 mg
EUR 265.74
Description: C28H35N3O4

Paraherquamide E

P070-2.5MG 2.5 mg
EUR 928.58
Description: C28H35N3O4

Phenelfamycin E

P078-1MG 1 mg
EUR 207.65
Description: C65H95NO21

Phenelfamycin E

P078-5MG 5 mg
EUR 726.13
Description: C65H95NO21

Roquefortine E

R035-1MG 1 mg
EUR 146.96
Description: C27H31N5O2

Roquefortine E

R035-5MG 5 mg
EUR 513.71
Description: C27H31N5O2

Azithromycin impurity E EvoPure®

A074-5MG 5 mg
EUR 593.04
Description: C36H68N2O12

Hepatitis E virus ORF 2 recombinant antigen

8942 100 ug
EUR 477.6
Description: This is Hepatitis E virus ORF 2 recombinant antigen for ELISA,WB.

Hepatitis E virus ORF 2 recombinant antigen

8943 100 ug
EUR 477.6
Description: This is Hepatitis E virus ORF 2 recombinant antigen for ELISA,WB.

Hepatitis E virus ORF 3 recombinant antigen

8944 100 ug
EUR 477.6
Description: This is Hepatitis E virus ORF 3 recombinant antigen for ELISA,WB.

Hepatitis E virus ORF 3 Recombinant Protein

53-335 0.01 mg
EUR 651.3
Description: Hepatitis E virus ORF 3 Recombinant Protein

Hepatitis E virus ORF 2 Recombinant Protein

53-336 0.01 mg
EUR 651.3
Description: Hepatitis E virus ORF 2 Recombinant Protein

Hepatitis E virus ORF 2 Recombinant Protein

53-337 0.01 mg
EUR 651.3
Description: Hepatitis E virus ORF 2 Recombinant Protein

Vascular Endothelial Growth Factor-E (Orf Virus) Protein

20-abx263083
  • Ask for price
  • Ask for price
  • Ask for price
  • 10 ug
  • 1 mg
  • 2 µg

Vascular Endothelial Growth Factor-E (Orf Virus) Protein

abx263083-10mg 10 mg
EUR 325

Vascular Endothelial Growth Factor-E (Orf Virus) Protein

abx263083-25mg 25 mg
EUR 6500

Vascular Endothelial Growth Factor-E (Orf Virus) Protein

abx263083-5mg 5 mg
EUR 225

Recombinant Vaccinia virus Uncharacterized 7.6 kDa protein (E ORF E)

MBS1007472-002mgBaculovirus 0.02mg(Baculovirus)
EUR 990

Recombinant Vaccinia virus Uncharacterized 7.6 kDa protein (E ORF E)

MBS1007472-002mgEColi 0.02mg(E-Coli)
EUR 555

Recombinant Vaccinia virus Uncharacterized 7.6 kDa protein (E ORF E)

MBS1007472-002mgYeast 0.02mg(Yeast)
EUR 745

Recombinant Vaccinia virus Uncharacterized 7.6 kDa protein (E ORF E)

MBS1007472-01mgEColi 0.1mg(E-Coli)
EUR 660

Recombinant Vaccinia virus Uncharacterized 7.6 kDa protein (E ORF E)

MBS1007472-01mgYeast 0.1mg(Yeast)
EUR 870

Vascular Endothelial Growth Factor-E (Orf Virus), (Recombinant)

22060748-1 2 µg
EUR 146.42

Recombinant Vascular Endothelial Growth Factor-E (Orf Virus)

7-01759 2µg Ask for price

Recombinant Vascular Endothelial Growth Factor-E (Orf Virus)

7-01760 10µg Ask for price

Recombinant Vascular Endothelial Growth Factor-E (Orf Virus)

7-01761 1mg Ask for price

Vascular Endothelial Growth Factor-E Recombinant (Orf Virus)

rAP-2496 Inquiry Ask for price

Recombinant Vascular Endothelial Growth Factor-E (Orf Virus)

cyt-263-10g 10µg
EUR 145

Recombinant Vascular Endothelial Growth Factor-E (Orf Virus)

cyt-263-1mg 1mg
EUR 5100

Recombinant Vascular Endothelial Growth Factor-E (Orf Virus)

cyt-263-2g 2µg
EUR 60

Recombinant Vascular Endothelial Growth Factor-E (Orf Virus)

MBS142140-0002mg 0.002mg
EUR 240

Recombinant Vascular Endothelial Growth Factor-E (Orf Virus)

MBS142140-001mg 0.01mg
EUR 310

Recombinant Vascular Endothelial Growth Factor-E (Orf Virus)

MBS142140-1mg 1mg
EUR 5325

Recombinant Vascular Endothelial Growth Factor-E (Orf Virus)

MBS142140-5x1mg 5x1mg
EUR 23640

Hepatitis E Virus ORF3 Protein

20-abx260138
  • Ask for price
  • Ask for price
  • Ask for price
  • 0.5 mg
  • 100 ug
  • 1 mg

Hepatitis E Virus ORF3 Protein

abx260138-20g 20 µg
EUR 950

Hepatitis E Virus ORF3 Protein

abx260138-5g 5 µg
EUR 350

Hepatitis E virus

PCR-H498-PCRH49848R PCR-H498-48R
EUR 485

Hepatitis E virus

PCR-H498-PCRH49896R PCR-H498-96R
EUR 685

Hepatitis E virus

Oneq-H498-OneqH498100R Oneq-H498-100R
EUR 1028

Hepatitis E virus

Oneq-H498-OneqH498150R Oneq-H498-150R
EUR 1438

Hepatitis E virus

Oneq-H498-OneqH49850R Oneq-H498-50R
EUR 728

Hepatitis E virus

MBS328020-01mg 0.1mg
EUR 435

Hepatitis E virus

MBS328020-5x01mg 5x0.1mg
EUR 1865

Hepatitis E virus

MBS328021-01mg 0.1mg
EUR 435

Hepatitis E virus

MBS328021-5x01mg 5x0.1mg
EUR 1865

Hepatitis E virus

MBS328022-01mg 0.1mg
EUR 435

Hepatitis E virus

MBS328022-5x01mg 5x0.1mg
EUR 1865

Hepatitis E Virus ORF3 (Recombinant)

22060181-1 100 µg
EUR 264.47

Recombinant Hepatitis E Virus ORF3 

7-07450 100µg Ask for price

Recombinant Hepatitis E Virus ORF3 

7-07451 500µg Ask for price

Recombinant Hepatitis E Virus ORF3 

7-07452 1000µg Ask for price

Hepatitis E Virus ORF3 Recombinant

rAP-5321 Inquiry Ask for price

Recombinant Hepatitis E Virus ORF3 

MBS142588-01mg 0.1mg
EUR 330

Recombinant Hepatitis E Virus ORF3 

MBS142588-05mg 0.5mg
EUR 825

Recombinant Hepatitis E Virus ORF3 

MBS142588-1mg 1mg
EUR 1515

Recombinant Hepatitis E Virus ORF3 

MBS142588-5x1mg 5x1mg
EUR 6485

Hepatitis E Virus, ORF2, Recombinant (HEV)

MBS637880-01mg 0.1mg
EUR 455

Hepatitis E Virus, ORF2, Recombinant (HEV)

MBS637880-05mg 0.5mg
EUR 1025

Hepatitis E Virus, ORF2, Recombinant (HEV)

MBS637880-5x05mg 5x0.5mg
EUR 4470

Hepatitis E Virus, ORF3, Recombinant (HEV)

MBS636730-01mg 0.1mg
EUR 735

Hepatitis E Virus, ORF3, Recombinant (HEV)

MBS636730-5x01mg 5x0.1mg
EUR 3155

VEGF (165): Human Recombinant E Coli

GWB-BDEFEF 1 mg Ask for price

OPPA00528-2UG - VEGF E Protein

OPPA00528-2UG 2ug
EUR 70

Hepatitis E virus (ratHEV)

Oneq-V372-OneqV372100R Oneq-V372-100R
EUR 1028

Hepatitis E virus (ratHEV)

Oneq-V372-OneqV372150R Oneq-V372-150R
EUR 1438

Hepatitis E virus (ratHEV)

Oneq-V372-OneqV37250R Oneq-V372-50R
EUR 728

Hepatitis E virus (ratHEV)

PCR-V372-PCRV37248R PCR-V372-48R
EUR 485

Hepatitis E virus (ratHEV)

PCR-V372-PCRV37296R PCR-V372-96R
EUR 685

Hepatitis E Virus ORF2 (452-617) Protein

20-abx260268
  • Ask for price
  • Ask for price
  • Ask for price
  • 0.5 mg
  • 100 ug
  • 1 mg

Hepatitis E Virus ORF2 (633-659) Protein

20-abx260269
  • Ask for price
  • Ask for price
  • Ask for price
  • 0.5 mg
  • 100 ug
  • 1 mg

Hepatitis E Virus ORF2 (403-461) Protein

20-abx260270
  • Ask for price
  • Ask for price
  • Ask for price
  • 0.5 mg
  • 100 ug
  • 1 mg

Hepatitis E Virus ORF2 (452-617) Protein

abx260268-20g 20 µg
EUR 950

Hepatitis E Virus ORF2 (452-617) Protein

abx260268-5g 5 µg
EUR 350

Hepatitis E Virus ORF2 (633-659) Protein

abx260269-25g 25 µg
EUR 950

Hepatitis E Virus ORF2 (633-659) Protein

abx260269-5g 5 µg
EUR 350

Hepatitis E Virus ORF2 (403-461) Protein

abx260270-25g 25 µg
EUR 950

Hepatitis E Virus ORF2 (403-461) Protein

abx260270-5g 5 µg
EUR 350