The MyFugeMini centrifuge is a personal centrifuge that virtually fits in the palm of your hand. Ideal for quick spin downs of microtubes and PCR tubes, the MyFuge is extremely easy to use. Simply close the lid and the rotor instantly reaches 6,000 rpm. Open the lid, and the rotor quickly decelerates to a stop for removing samples. An increased capacity, eight-position microtube rotor is included and comes installed. Also included is a second rotor for 0.2 ml PCR tubes … Continue reading „Konisches 2-ml-Röhrchen mit Schraubverschluss, steril, rot, 500/Beutel“
AccuCount Rainbow fluoreszierende Partikel
Posted on Juni 3, 2022Juni 4, 2022Categories Assay Kits, PCR Leave a comment on AccuCount Rainbow fluoreszierende PartikelAnalysis of single nanoparticles by flow cytometry involves the measurement of weak signals that are close to the instrument’s detection limits. Distinguishing the signal from particles of interest from background particles in buffers and from optical and electronic noise can be difficult and requires careful consideration of the measurement approach, control experiments, and examination of the resulting data. In applying this check, we have identified an artifact resulting from inappropriate trigger channel threshold selection that may not be obvious to … Continue reading „AccuCount Rainbow fluoreszierende Partikel“
SARS-CoV-2 Spike P681H Antikörper (Alpha, Mu-Variante)
Posted on Juni 3, 2022Juni 4, 2022Categories Assay Kits, RNA Leave a comment on SARS-CoV-2 Spike P681H Antikörper (Alpha, Mu-Variante)Since the first reported human infection with SARS-CoV-2 in December 2019, many protein subunit vaccines have been proposed for human use. Subunit vaccines Use one or more antigens suitable for eliciting a robust immune response. However, the main concern is the effectiveness of subunit vaccines and elicited antibodies to neutralize variants of SARS-CoV-2 such as B.1.1.7 (alpha), B.1.351 (beta) and P1 (gamma), B.1.617 ( Delta) and C.37 (Lambda). The spike protein (S) is a potential fragment for use as an antigen … Continue reading „SARS-CoV-2 Spike P681H Antikörper (Alpha, Mu-Variante)“
EBV-Glykoprotein 125
Posted on Juni 3, 2022Juni 4, 2022Categories Assay Kits, RNA Leave a comment on EBV-Glykoprotein 125The Epstein-Barr virus (EBV) homologue of the conserved herpesvirus glycoprotein gN is predicted to be encoded by the BLRF1 open reading frame (ORF). Antipeptide antibodies against a sequence corresponding to residues in the predicted BLRF1 ORF immunoprecipitated an approximately 8 kDa doublet from cells expressing the BLRF1 ORF as a recombinant protein. In addition, four glycosylated proteins of 113, 84, 48, and 15 kDa from virus-producing cells could be immunoprecipitated by the same antibody. The 15 kDa species was the … Continue reading „EBV-Glykoprotein 125“
HIV-1 p24 rekombinantes Antigen
Posted on Juni 3, 2022Juni 4, 2022Categories Assay Kits, Pcr Kits Leave a comment on HIV-1 p24 rekombinantes AntigenDas humane Immunschwächevirus Typ 1 (HIV-1) ist die Hauptursache des erworbenen Immunschwächesyndroms (AIDS)[ ]. Die Diagnose einer HIV-Infektion, insbesondere die Früherkennung, ist ein wichtiger Teil der AIDS-Prävention und -Kontrolle[ ]. Das Gag-Protein von HIV-1, ein Polyprotein von 55 kDa, ist eines der am stärksten konservierten viralen Proteine. Das Gag-Protein wird durch eine virale Protease gespalten, um p17, p24 und p12 während der viralen Reifung freizusetzen[ ]. Das P24-Protein ist das wichtigste Kernprotein des Viruspartikels und wurde als spezifisches Ziel für antivirale Strategien vorgeschlagen[ ]. Das P24-Protein ist … Continue reading „HIV-1 p24 rekombinantes Antigen“
Malachitgrüner konjugierter Antikörper
Posted on Juni 3, 2022Juni 4, 2022Categories Panel, RNA Leave a comment on Malachitgrüner konjugierter AntikörperWe report that a symmetric small molecule ligand mediates assembly of the antibody light chain variable domains (V L s) into a corresponding symmetric ternary complex with novel interfaces. L5* Fluorogen Activating Protein (FAP) is a VL domain that binds malachite green (MG) dye to activate intense fluorescence. Crystallography of liganded L5* reveals a 2:1 protein:ligand complex with extensive C2 symmetry, with MG almost completely encapsulated between an antiparallel arrangement of the two VL domains. Unliganded L5* VL domains crystallize as … Continue reading „Malachitgrüner konjugierter Antikörper“
Rekombinantes menschliches CTRB1-Protein, ohne Tag, E. coli-50 mg
Posted on Juni 3, 2022Juni 4, 2022Categories Panel, Pcr Kits Leave a comment on Rekombinantes menschliches CTRB1-Protein, ohne Tag, E. coli-50 mgDie posttranslationale Modifikation durch kleine Ubiquitin-ähnliche Modifikatoren (SUMO) wird mit einer wachsenden Zahl regulatorischer Funktionen in verschiedenen zellulären Prozessen in Verbindung gebracht. Die biochemische Untersuchung der zugrunde liegenden molekularen Mechanismen hinkt jedoch hinterher, da es schwierig ist, ausreichende Mengen rekombinanter SUMOylierter Proteine herzustellen. Hier stellen wir zwei neu entwickelte Zweikomponenten-Vektorsysteme für die Expression und Reinigung von SUMO-modifizierten Zielproteinen in Escherichia coli vor . Ein System besteht aus einem Vektor für die SUMO-Konjugation, der humane SUMO-aktivierende (SAE1/SAE2) und konjugierende (Ubc9) Enzyme zusammen mit His 6 exprimiert-getaggte SUMO1, … Continue reading „Rekombinantes menschliches CTRB1-Protein, ohne Tag, E. coli-50 mg“
Human Insulin Like Growth Factor 1 (IGF1)
Posted on Juni 3, 2022Juni 4, 2022Categories Assay Kits, Panel Leave a comment on Human Insulin Like Growth Factor 1 (IGF1)Insulin-like growth factor 1 (IGF1) is an important biomarker for the treatment of growth hormone disorders. Recently, there has been increased interest in using mass spectrometric (MS) detection methods to measure IGF1. However, widespread clinical deployment of an MS-based IGF1 assay requires increased throughput and speed to justify analysis costs, as well as robust industrial platforms that are reproducible across laboratories. Presented here is an MS-based quantitative IGF1 assay with a performance evaluation of >1,000 samples/day and an ability to … Continue reading „Human Insulin Like Growth Factor 1 (IGF1)“
MCDB 131 W/O L-Glutamin
Posted on Juni 3, 2022Juni 4, 2022Categories Assay Kits, RNA Leave a comment on MCDB 131 W/O L-GlutaminProtocols for maintenance of hPSCs and generation of lung cells were slightly modified from previous studies (Chen et al., 2019; Huang et al., 2014). The hESC line-RUES2 was cultured on irradiated mouse embryonic fibroblasts (Global Stem, cat. no. GSC-6001G) at a density of 20,000-25,000 cells/cm2 in a medium of DMEM/F12, 20% knockout serum replacement (Life Technologies ), 0.1 mM β-mercaptoethanol (Sigma Aldrich) and 20 ng/ml bFGF (R&D Systems), and medium was changed daily. hESC cultures were maintained in an undifferentiated … Continue reading „MCDB 131 W/O L-Glutamin“
Hämoglobinprotein (Maus)
Posted on Juni 3, 2022Juni 4, 2022Categories Assay Kits, RNA Leave a comment on Hämoglobinprotein (Maus)Insight into the evolution of hemoglobins and their genes is a rich source of ideas about the function of hemoglobin and the regulation of globin gene expression. This article introduces the numerous genes and gene families encoding human globins, summarizes important events in the evolution of hemoglobin gene clusters, and discusses how these studies provide insight into the regulation of globin genes. Although the genes in and around the α-like globin gene complex are relatively stable, the β-like globin gene … Continue reading „Hämoglobinprotein (Maus)“