Assay Kits – Convertex Chemie GmbH – Herstellung von Spezialchemikalien

Konisches 2-ml-Röhrchen mit Schraubverschluss, steril, rot, 500/Beutel

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The MyFugeMini centrifuge is a personal centrifuge that virtually fits in the palm of your hand. Ideal for quick spin downs of microtubes and PCR tubes, the MyFuge is extremely easy to use. Simply close the lid and the rotor instantly reaches 6,000 rpm. Open the lid, and the rotor quickly decelerates to a stop for removing samples. An increased capacity, eight-position microtube rotor is included and comes installed. Also included is a second rotor for 0.2 ml PCR tubes … Continue reading „Konisches 2-ml-Röhrchen mit Schraubverschluss, steril, rot, 500/Beutel“

AccuCount Rainbow fluoreszierende Partikel

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Analysis of single nanoparticles by flow cytometry involves the measurement of weak signals that are close to the instrument’s detection limits. Distinguishing the signal from particles of interest from background particles in buffers and from optical and electronic noise can be difficult and requires careful consideration of the measurement approach, control experiments, and examination of the resulting data. In applying this check, we have identified an artifact resulting from inappropriate trigger channel threshold selection that may not be obvious to … Continue reading „AccuCount Rainbow fluoreszierende Partikel“

SARS-CoV-2 Spike P681H Antikörper (Alpha, Mu-Variante)

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Since the first reported human infection with SARS-CoV-2 in December 2019, many protein subunit vaccines have been proposed for human use. Subunit vaccines Use one or more antigens suitable for eliciting a robust immune response. However, the main concern is the effectiveness of subunit vaccines and elicited antibodies to neutralize variants of SARS-CoV-2 such as B.1.1.7 (alpha), B.1.351 (beta) and P1 (gamma), B.1.617 ( Delta) and C.37 (Lambda). The spike protein (S) is a potential fragment for use as an antigen … Continue reading „SARS-CoV-2 Spike P681H Antikörper (Alpha, Mu-Variante)“

EBV-Glykoprotein 125

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The Epstein-Barr virus (EBV) homologue of the conserved herpesvirus glycoprotein gN is predicted to be encoded by the BLRF1 open reading frame (ORF). Antipeptide antibodies against a sequence corresponding to residues in the predicted BLRF1 ORF immunoprecipitated an approximately 8 kDa doublet from cells expressing the BLRF1 ORF as a recombinant protein. In addition, four glycosylated proteins of 113, 84, 48, and 15 kDa from virus-producing cells could be immunoprecipitated by the same antibody. The 15 kDa species was the … Continue reading „EBV-Glykoprotein 125“

HIV-1 p24 rekombinantes Antigen

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Das humane Immunschwächevirus Typ 1 (HIV-1) ist die Hauptursache des erworbenen Immunschwächesyndroms (AIDS)[ ]. Die Diagnose einer HIV-Infektion, insbesondere die Früherkennung, ist ein wichtiger Teil der AIDS-Prävention und -Kontrolle[ ]. Das Gag-Protein von HIV-1, ein Polyprotein von 55 kDa, ist eines der am stärksten konservierten viralen Proteine. Das Gag-Protein wird durch eine virale Protease gespalten, um p17, p24 und p12 während der viralen Reifung freizusetzen[ ]. Das P24-Protein ist das wichtigste Kernprotein des Viruspartikels und wurde als spezifisches Ziel für antivirale Strategien vorgeschlagen[ ]. Das P24-Protein ist … Continue reading „HIV-1 p24 rekombinantes Antigen“

Human Insulin Like Growth Factor 1 (IGF1)

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Insulin-like growth factor 1 (IGF1) is an important biomarker for the treatment of growth hormone disorders. Recently, there has been increased interest in using mass spectrometric (MS) detection methods to measure IGF1. However, widespread clinical deployment of an MS-based IGF1 assay requires increased throughput and speed to justify analysis costs, as well as robust industrial platforms that are reproducible across laboratories. Presented here is an MS-based quantitative IGF1 assay with a performance evaluation of >1,000 samples/day and an ability to … Continue reading „Human Insulin Like Growth Factor 1 (IGF1)“

MCDB 131 W/O L-Glutamin

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Protocols for maintenance of hPSCs and generation of lung cells were slightly modified from previous studies (Chen et al., 2019; Huang et al., 2014). The hESC line-RUES2 was cultured on irradiated mouse embryonic fibroblasts (Global Stem, cat. no. GSC-6001G) at a density of 20,000-25,000 cells/cm2 in a medium of DMEM/F12, 20% knockout serum replacement (Life Technologies ), 0.1 mM β-mercaptoethanol (Sigma Aldrich) and 20 ng/ml bFGF (R&D Systems), and medium was changed daily. hESC cultures were maintained in an undifferentiated … Continue reading „MCDB 131 W/O L-Glutamin“

Hämoglobinprotein (Maus)

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Insight into the evolution of hemoglobins and their genes is a rich source of ideas about the function of hemoglobin and the regulation of globin gene expression. This article introduces the numerous genes and gene families encoding human globins, summarizes important events in the evolution of hemoglobin gene clusters, and discusses how these studies provide insight into the regulation of globin genes. Although the genes in and around the α-like globin gene complex are relatively stable, the β-like globin gene … Continue reading „Hämoglobinprotein (Maus)“

DNA Replication Regulator MCM Advanced Expression and Prognostic Significance in Hepatic

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Paternal DNA Methylation May Be Associated With Gestational Age at Begin Background: How epigenetic modifications of DNA are associated to gestational age at supply is simply not completely understood. We investigated potential outcomes of differential paternal DNA methylation (DNAm) on offspring gestational age at supply by conducting an epigenome-wide search for cytosine-phosphate-guanine (CpG) web sites. Methods: Analysis members on this analysis embody male cohort members or companions of the F1-generation of the Isle of Wight Begin Cohort (IoWBC). DNAm ranges in peripheral blood from F1-fathers (n = 92) collected spherical being pregnant of their spouses have been analyzed using the Illumina 450Okay array. A 5-step statistical analysis was carried out. First, a training-testing screening technique was utilized to pick CpG web … Continue reading „DNA Replication Regulator MCM Advanced Expression and Prognostic Significance in Hepatic“

DNA testing by routinely and sequentially performing extraction, amplification

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Fluorescent Lactic Acid Bacteria and Bifidobacteria as Vehicles of DNA Microbial Biosensors Management and quantification of effector molecules corresponding to heavy metals, toxins or different goal molecules is of nice biotechnological, social and financial curiosity. Microorganisms have regulatory proteins that acknowledge and modify the gene expression within the presence or absence of those compounds (effector molecules) via binding to gene sequences. The affiliation of those recognizing gene sequences to reporter genes will permit the detection of effector molecules of curiosity … Continue reading „DNA testing by routinely and sequentially performing extraction, amplification“