Fluorescent Lactic Acid Bacteria and Bifidobacteria as Vehicles of DNA Microbial Biosensors
Management and quantification of effector molecules corresponding to heavy metals, toxins or different goal molecules is of nice biotechnological, social and financial curiosity. Microorganisms have regulatory proteins that acknowledge and modify the gene expression within the presence or absence of those compounds (effector molecules) via binding to gene sequences. The affiliation of those recognizing gene sequences to reporter genes will permit the detection of effector molecules of curiosity with excessive sensitivity.
As soon as investigators have these two elements-recognizing gene sequences and reporter genes that emit signals-we want an appropriate automobile to introduce each components. Right here, we propose lactic acid micro organism (LAB) and bifidobacteria as promising service microorganisms for these molecular biosensors. Using fluorescent proteins in addition to food-grade vectors and clustered repeatedly interspaced brief palindromic repeats (CRISPR) are indispensable instruments for introducing biosensors into these microorganisms. Using these LAB and bifidobacteria can be of particular curiosity for learning the intestinal setting or different advanced ecosystems.
The good number of species tailored to many environments, in addition to the opportunity of making use of a number of protocols for his or her transformation with recognizing gene sequences and reporter genes are appreciable benefits. Lastly, an effort should be made to search out recognizable gene sequences.
DNAAF5 Polyclonal Antibody |
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| A58814 |
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DNAAF5 Antibody, HRP conjugated |
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| 1-CSB-PA771471LB01HU |
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Description: A polyclonal antibody against DNAAF5. Recognizes DNAAF5 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
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DNAAF5 Antibody, FITC conjugated |
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| 1-CSB-PA771471LC01HU |
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Description: A polyclonal antibody against DNAAF5. Recognizes DNAAF5 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA |
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DNAAF5 Antibody, Biotin conjugated |
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| 1-CSB-PA771471LD01HU |
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Description: A polyclonal antibody against DNAAF5. Recognizes DNAAF5 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA |
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DNAAF5 Polyclonal Antibody, HRP Conjugated |
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| A58817 |
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DNAAF5 Polyclonal Antibody, FITC Conjugated |
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| A58816 |
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DNAAF5 Polyclonal Antibody, Biotin Conjugated |
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| A58815 |
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Human Dynein Assembly Factor 5, Axonemal / HEATR2 (DNAAF5) ELISA Kit |
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| abx387761-96tests | 96 tests | EUR 1093.2 |
DNAAF3 Antibody |
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| 20-abx313748 |
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DNAAF1 antibody |
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| 10R-6890 | 100 ul | EUR 829.2 |
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Description: Mouse monoclonal DNAAF1 antibody |
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DNAAF1 antibody |
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| 10R-6892 | 100 ul | EUR 829.2 |
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Description: Mouse monoclonal DNAAF1 antibody |
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DNAAF1 antibody |
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| 10R-6893 | 100 ul | EUR 829.2 |
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Description: Mouse monoclonal DNAAF1 antibody |
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DNAAF3 Antibody |
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| 1-CSB-PA850845LA01HU |
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Description: A polyclonal antibody against DNAAF3. Recognizes DNAAF3 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC, IF; Recommended dilution: IHC:1:20-1:200, IF:1:50-1:200 |
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DNAAF4 Antibody |
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| 1-CSB-PA855504LA01HU |
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Description: A polyclonal antibody against DNAAF4. Recognizes DNAAF4 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
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DNAAF1 Antibody |
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| DF14672 | 100ul | EUR 280 |
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Description: Human,Mouse,Rat |
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DNAAF2 Antibody |
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| E40PV8515 | 50ul | EUR 395 |
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Description: Available in various conjugation types. |
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DNAAF1 Antibody |
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| DF14672-100ul | 100ul | EUR 280 |
DNAAF1 Antibody |
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| DF14672-200ul | 200ul | EUR 350 |
DNAAF3 Antibody (HRP) |
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| 20-abx313749 |
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DNAAF3 Antibody (FITC) |
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| 20-abx313750 |
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DNAAF3 Antibody (Biotin) |
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| 20-abx313751 |
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DNAAF3 Antibody, HRP conjugated |
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| 1-CSB-PA850845LB01HU |
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Description: A polyclonal antibody against DNAAF3. Recognizes DNAAF3 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
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DNAAF4 Antibody, HRP conjugated |
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| 1-CSB-PA855504LB01HU |
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Description: A polyclonal antibody against DNAAF4. Recognizes DNAAF4 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
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DNAAF3 Antibody, FITC conjugated |
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| 1-CSB-PA850845LC01HU |
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Description: A polyclonal antibody against DNAAF3. Recognizes DNAAF3 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA |
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DNAAF4 Antibody, FITC conjugated |
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| 1-CSB-PA855504LC01HU |
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Description: A polyclonal antibody against DNAAF4. Recognizes DNAAF4 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA |
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DNAAF3 Antibody, Biotin conjugated |
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| 1-CSB-PA850845LD01HU |
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Description: A polyclonal antibody against DNAAF3. Recognizes DNAAF3 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA |
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DNAAF4 Antibody, Biotin conjugated |
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| 1-CSB-PA855504LD01HU |
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Description: A polyclonal antibody against DNAAF4. Recognizes DNAAF4 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA |
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DNAAF1 Polyclonal Antibody |
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| 29897-100ul | 100ul | EUR 302.4 |
DNAAF1 Polyclonal Antibody |
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| 29897-50ul | 50ul | EUR 224.4 |
DNAAF1 Polyclonal Antibody |
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| E916593 | 100ul | EUR 225 |
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Description: Available in various conjugation types. |
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DNAAF1 Polyclonal Conjugated Antibody |
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| C29897 | 100ul | EUR 476.4 |
Dyslexia Susceptibility 1 Candidate 1 / DYX1C1 (DNAAF4) Antibody |
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| 20-abx318218 |
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Dyslexia Susceptibility 1 Candidate 1 / DYX1C1 (DNAAF4) Antibody |
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| 20-abx112209 |
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Dyslexia Susceptibility 1 Candidate 1 / DYX1C1 (DNAAF4) Antibody (HRP) |
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| 20-abx315110 |
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Dyslexia Susceptibility 1 Candidate 1 / DYX1C1 (DNAAF4) Antibody (FITC) |
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| 20-abx315111 |
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Dyslexia Susceptibility 1 Candidate 1 / DYX1C1 (DNAAF4) Antibody (Biotin) |
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| 20-abx315112 |
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Dnaaf1/ Rat Dnaaf1 ELISA Kit |
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| ELI-50643r | 96 Tests | EUR 1063.2 |
Dnaaf2/ Rat Dnaaf2 ELISA Kit |
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| ELI-08767r | 96 Tests | EUR 1063.2 |
DNAAF1 siRNA |
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| 20-abx914304 |
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DNAAF1 siRNA |
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| 20-abx914305 |
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DNAAF2 siRNA |
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| 20-abx914306 |
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DNAAF2 siRNA |
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| 20-abx914307 |
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DNAAF3 siRNA |
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| 20-abx914308 |
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DNAAF3 siRNA |
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| 20-abx914309 |
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DNAAF1 siRNA |
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| 20-abx901521 |
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DNAAF2 siRNA |
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| 20-abx901522 |
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DNAAF3 siRNA |
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| 20-abx901523 |
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DNAAF1 Rabbit pAb |
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| A16593-100ul | 100 ul | EUR 369.6 |
DNAAF1 Rabbit pAb |
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| A16593-200ul | 200 ul | EUR 550.8 |
DNAAF1 Rabbit pAb |
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| A16593-20ul | 20 ul | EUR 219.6 |
DNAAF1 Rabbit pAb |
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| A16593-50ul | 50 ul | EUR 267.6 |
DNAAF1 ELISA KIT|Human |
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| EF004885 | 96 Tests | EUR 826.8 |
Dnaaf1 ORF Vector (Rat) (pORF) |
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| ORF066087 | 1.0 ug DNA | EUR 607.2 |
Dnaaf2 ORF Vector (Rat) (pORF) |
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| ORF066088 | 1.0 ug DNA | EUR 607.2 |
Rat DNAAF3 shRNA Plasmid |
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| 20-abx991254 |
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Rat DNAAF1 shRNA Plasmid |
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| 20-abx990192 |
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Rat DNAAF2 shRNA Plasmid |
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| 20-abx990437 |
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DNAAF1 ORF Vector (Human) (pORF) |
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| ORF018393 | 1.0 ug DNA | Ask for price |
DNAAF2 ORF Vector (Human) (pORF) |
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| ORF018394 | 1.0 ug DNA | Ask for price |
DNAAF3 ORF Vector (Human) (pORF) |
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| ORF018395 | 1.0 ug DNA | Ask for price |
Dnaaf1 3'UTR GFP Stable Cell Line |
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| TU253484 | 1.0 ml | Ask for price |
Dnaaf2 3'UTR GFP Stable Cell Line |
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| TU253485 | 1.0 ml | Ask for price |
Mouse DNAAF1 shRNA Plasmid |
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| 20-abx976562 |
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Human DNAAF1 shRNA Plasmid |
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| 20-abx964742 |
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Mouse DNAAF3 shRNA Plasmid |
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| 20-abx984074 |
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Human DNAAF2 shRNA Plasmid |
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| 20-abx960524 |
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Mouse DNAAF2 shRNA Plasmid |
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| 20-abx979964 |
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Human DNAAF3 shRNA Plasmid |
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| 20-abx967554 |
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Dnaaf1 Protein Vector (Rat) (pPM-C-HA) |
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| PV264348 | 500 ng | EUR 723.6 |
Dnaaf2 Protein Vector (Rat) (pPM-C-HA) |
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| PV264352 | 500 ng | EUR 1399.2 |
Human DNAAF1 Protein Lysate 20ug |
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| IHUDNAAF1PLLY20UG | each | EUR 213 |
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Description: Human DNAAF1 Protein Lysate 20ug |
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Human DNAAF2 Protein Lysate 20ug |
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| IHUDNAAF2PLLY20UG | each | EUR 361 |
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Description: Human DNAAF2 Protein Lysate 20ug |
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Human DNAAF3 Protein Lysate 20ug |
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| IHUDNAAF3PLLY20UG | each | EUR 361 |
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Description: Human DNAAF3 Protein Lysate 20ug |
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Human DNAAF4 Protein Lysate 20ug |
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| IHUDNAAF4PLLY20UG | each | EUR 213 |
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Description: Human DNAAF4 Protein Lysate 20ug |
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Dnaaf1 Recombinant Protein (Rat) |
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| RP198257 | 100 ug | Ask for price |
Dnaaf2 Recombinant Protein (Rat) |
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| RP198260 | 100 ug | Ask for price |
Dnaaf1 Protein Vector (Rat) (pPB-C-His) |
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| PV264346 | 500 ng | EUR 723.6 |
Dnaaf1 Protein Vector (Rat) (pPB-N-His) |
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| PV264347 | 500 ng | EUR 723.6 |
Dnaaf1 Protein Vector (Rat) (pPM-C-His) |
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| PV264349 | 500 ng | EUR 723.6 |
Dnaaf2 Protein Vector (Rat) (pPB-C-His) |
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| PV264350 | 500 ng | EUR 1399.2 |
Dnaaf2 Protein Vector (Rat) (pPB-N-His) |
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| PV264351 | 500 ng | EUR 1399.2 |
Dnaaf2 Protein Vector (Rat) (pPM-C-His) |
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| PV264353 | 500 ng | EUR 1399.2 |
Dnaaf1 Protein Vector (Human) (pPM-C-HA) |
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| PV073571 | 500 ng | Ask for price |
Dnaaf2 Protein Vector (Human) (pPM-C-HA) |
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| PV073575 | 500 ng | Ask for price |
DNAAF3 Protein Vector (Human) (pPM-C-HA) |
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| PV073579 | 500 ng | Ask for price |
Human Protein kintoun, DNAAF2 ELISA KIT |
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| ELI-47983h | 96 Tests | EUR 988.8 |
Mouse Protein kintoun, Dnaaf2 ELISA KIT |
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| ELI-21095m | 96 Tests | EUR 1038 |
Dnaaf1 Protein Vector (Human) (pPB-C-His) |
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| PV073569 | 500 ng | Ask for price |
Dnaaf1 Protein Vector (Human) (pPB-N-His) |
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| PV073570 | 500 ng | Ask for price |
Dnaaf1 Protein Vector (Human) (pPM-C-His) |
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| PV073572 | 500 ng | Ask for price |
Dnaaf2 Protein Vector (Human) (pPB-C-His) |
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| PV073573 | 500 ng | Ask for price |
Dnaaf2 Protein Vector (Human) (pPB-N-His) |
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| PV073574 | 500 ng | Ask for price |
Dnaaf2 Protein Vector (Human) (pPM-C-His) |
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| PV073576 | 500 ng | Ask for price |
DNAAF3 Protein Vector (Human) (pPB-C-His) |
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| PV073577 | 500 ng | Ask for price |
DNAAF3 Protein Vector (Human) (pPB-N-His) |
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| PV073578 | 500 ng | Ask for price |
DNAAF3 Protein Vector (Human) (pPM-C-His) |
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| PV073580 | 500 ng | Ask for price |
Dnaaf1 Recombinant Protein (Human) |
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| RP055176 | 100 ug | Ask for price |
Dnaaf2 Recombinant Protein (Human) |
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| RP055179 | 100 ug | Ask for price |
DNAAF3 Recombinant Protein (Human) |
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| RP055182 | 100 ug | Ask for price |
Dnaaf1 3'UTR Luciferase Stable Cell Line |
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| TU203484 | 1.0 ml | Ask for price |
Dnaaf2 3'UTR Luciferase Stable Cell Line |
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| TU203485 | 1.0 ml | Ask for price |
Dnaaf2 sgRNA CRISPR Lentivector set (Rat) |
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| K7593601 | 3 x 1.0 ug | EUR 406.8 |
Dnaaf1 sgRNA CRISPR Lentivector set (Rat) |
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| K7566701 | 3 x 1.0 ug | EUR 406.8 |
Bovine Protein kintoun, DNAAF2 ELISA KIT |
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| ELI-43337b | 96 Tests | EUR 1113.6 |
Dnaaf2 sgRNA CRISPR Lentivector (Rat) (Target 1) |
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| K7593602 | 1.0 ug DNA | EUR 184.8 |
Dnaaf2 sgRNA CRISPR Lentivector (Rat) (Target 2) |
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| K7593603 | 1.0 ug DNA | EUR 184.8 |
Dnaaf2 sgRNA CRISPR Lentivector (Rat) (Target 3) |
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| K7593604 | 1.0 ug DNA | EUR 184.8 |
Dnaaf1 sgRNA CRISPR Lentivector (Rat) (Target 1) |
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| K7566702 | 1.0 ug DNA | EUR 184.8 |
Dnaaf1 sgRNA CRISPR Lentivector (Rat) (Target 2) |
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| K7566703 | 1.0 ug DNA | EUR 184.8 |
Dnaaf1 sgRNA CRISPR Lentivector (Rat) (Target 3) |
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| K7566704 | 1.0 ug DNA | EUR 184.8 |
Rat Dynein assembly factor 1, axonemal (DNAAF1) ELISA Kit |
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| abx391264-96tests | 96 tests | EUR 1093.2 |
Dnaaf2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV) |
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| LV654319 | 1.0 ug DNA | EUR 1626 |
Dnaaf2 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC) |
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| LV654323 | 1.0 ug DNA | EUR 1626 |
Dnaaf1 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV) |
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| LV680203 | 1.0 ug DNA | EUR 818.4 |
Dnaaf1 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC) |
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| LV680207 | 1.0 ug DNA | EUR 818.4 |
Dnaaf2 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat) |
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| K7593605 | 3 x 1.0 ug | EUR 451.2 |
Dnaaf1 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat) |
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| K7566705 | 3 x 1.0 ug | EUR 451.2 |
Mouse Dynein assembly factor 1, axonemal (DNAAF1) ELISA Kit |
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| abx389121-96tests | 96 tests | EUR 1093.2 |
Human Dynein assembly factor 1, axonemal (DNAAF1) ELISA Kit |
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| abx384818-96tests | 96 tests | EUR 1093.2 |
Dnaaf2 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a) |
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| LV654324 | 1.0 ug DNA | EUR 1626 |
Dnaaf1 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a) |
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| LV680208 | 1.0 ug DNA | EUR 818.4 |
Dnaaf1 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV759989 | 1.0 ug DNA | EUR 1104 |
Dnaaf1 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV759993 | 1.0 ug DNA | EUR 1104 |
DNAAF3 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV759995 | 1.0 ug DNA | EUR 686.4 |
DNAAF3 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV759999 | 1.0 ug DNA | EUR 686.4 |
Human Dynein assembly factor 1, axonemal, DNAAF1 ELISA KIT |
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| ELI-50642h | 96 Tests | EUR 988.8 |
Mouse Dynein assembly factor 1, axonemal, Dnaaf1 ELISA KIT |
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| ELI-31900m | 96 Tests | EUR 1038 |
Bovine Dynein assembly factor 1, axonemal, DNAAF1 ELISA KIT |
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| ELI-26476b | 96 Tests | EUR 1113.6 |
Dnaaf1 ELISA Kit| Rat Dynein assembly factor 1, axonemal ELISA |
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| EF018619 | 96 Tests | EUR 826.8 |
Dnaaf2 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 1) |
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| K7593606 | 1.0 ug DNA | EUR 200.4 |
Dnaaf2 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 2) |
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| K7593607 | 1.0 ug DNA | EUR 200.4 |
Dnaaf2 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 3) |
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| K7593608 | 1.0 ug DNA | EUR 200.4 |
Dnaaf1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 1) |
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| K7566706 | 1.0 ug DNA | EUR 200.4 |
Dnaaf1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 2) |
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| K7566707 | 1.0 ug DNA | EUR 200.4 |
Dnaaf1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 3) |
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| K7566708 | 1.0 ug DNA | EUR 200.4 |
Dnaaf1 Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV759994 | 1.0 ug DNA | EUR 1104 |
DNAAF3 Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV760000 | 1.0 ug DNA | EUR 686.4 |
DNAAF1 ELISA Kit| Bovine Dynein assembly factor 1, axonemal ELI |
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| EF011338 | 96 Tests | EUR 826.8 |
Dnaaf1 ELISA Kit| Mouse Dynein assembly factor 1, axonemal ELIS |
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| EF014751 | 96 Tests | EUR 826.8 |
Dnaaf2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-C-term-HA) |
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| LV654320 | 1.0 ug DNA | EUR 1626 |
Dnaaf1 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-C-term-HA) |
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| LV680204 | 1.0 ug DNA | EUR 818.4 |
Dnaaf2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro) |
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| LV654321 | 1.0 ug DNA | EUR 1695.6 |
Dnaaf2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro) |
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| LV654322 | 1.0 ug DNA | EUR 1695.6 |
Dnaaf1 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro) |
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| LV680205 | 1.0 ug DNA | EUR 888 |
Dnaaf1 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro) |
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| LV680206 | 1.0 ug DNA | EUR 888 |
Dnaaf1 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-C-term-HA) |
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| LV759990 | 1.0 ug DNA | EUR 1104 |
DNAAF3 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-C-term-HA) |
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| LV759996 | 1.0 ug DNA | EUR 686.4 |
Human Dyslexia Susceptibility 1 Candidate 1 / DYX1C1 (DNAAF4) ELISA Kit |
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| abx387025-96tests | 96 tests | EUR 1093.2 |
Dnaaf1 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro) |
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| LV759991 | 1.0 ug DNA | EUR 1173.6 |
Dnaaf1 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro) |
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| LV759992 | 1.0 ug DNA | EUR 1173.6 |
DNAAF3 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro) |
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| LV759997 | 1.0 ug DNA | EUR 756 |
DNAAF3 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro) |
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| LV759998 | 1.0 ug DNA | EUR 756 |
DNA2 antibody |
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| 70R-16865 | 50 ul | EUR 522 |
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Description: Rabbit polyclonal DNA2 antibody |
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DNA2 Antibody |
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| 1-CSB-PA006982GA01HU |
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Description: A polyclonal antibody against DNA2. Recognizes DNA2 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC |
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DNA2 Antibody |
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| 1-CSB-PA006982LA01HU |
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Description: A polyclonal antibody against DNA2. Recognizes DNA2 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200 |
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DNA2 antibody |
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| PAab10066 | 100 ug | EUR 463.2 |
DNA2 Antibody |
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| E307839 | 200ul | EUR 275 |
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Description: Available in various conjugation types. |
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DNA2 Antibody |
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| CAC11831-100ug | 100ug | EUR 314 |
DNA2 Antibody |
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| CAC11831-50ug | 50ug | EUR 199.2 |
DNA2 antibody |
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| CAF50498-100ug | 100ug | EUR 338 |
Irregular Homocysteine Metabolism: An Perception of Alzheimer’s Illness from DNA Methylation
Alzheimer’s illness (AD) is a persistent neurodegenerative illness within the central nervous system that has advanced pathogenesis within the aged. The present evaluation focuses on the epigenetic mechanisms of AD, in response to the newest findings. The most effective-characterized chromatin modifications in epigenetic mechanisms is DNA methylation. Extremely replicable knowledge reveals that AD incidence is usually accompanied by methylation degree adjustments of the AD-related gene.
Homocysteine (Hcy) is just not solely an intermediate product of one-carbon metabolism but in addition an vital impartial danger issue of AD; it may possibly have an effect on the cognitive perform of the mind by altering the one-carbon metabolism and interfering with the DNA methylation course of, leading to cerebrovascular illness. Usually, Hcy could also be an environmental issue that impacts AD by way of the DNA methylation pathway with a collection of adjustments in AD-related substance. This evaluation will think about the relation between DNA methylation and Hcy and take a look at to determine their rule within the pathophysiology of AD.
TET is targeted for proteasomal degradation by the PHD-pVHL pathway to reduce DNA hydroxymethylation
Hypoxia-inducible components are heterodimeric transcription components that play an important position in a cell’s capability to adapt to low oxygen. The von-Hippel Lindau tumor suppressor (pVHL), acts as a grasp regulator of HIF exercise, and its focusing on of prolyl hydroxylated HIF-α for proteasomal degradation beneath normoxia is considered a significant mechanism for pVHL tumor suppression and mobile response to oxygen.
Whether or not pVHL regulates different targets by the same mechanism is essentially unknown. Right here, we determine TET2/Three as novel targets of pVHL. pVHL induces proteasomal degradation of TET2/3, leading to decreased international 5-hydroxymethylcytosine ranges.
Conserved proline residues inside the LAP/LAP-like motifs of those two proteins are hydroxylated by the prolyl hydroxylase enzymes (PHD2/EGLN1 and PHD3/EGLN3), which is prerequisite for pVHL-mediated degradation. Utilizing zebrafish as a mannequin, we decided that international 5-hydroxymethylcytosine ranges are enhanced in vhl-null, egln1a/b-double null and egln3-null embryos.
Subsequently, we reveal a novel perform for the PHD-pVHL pathway in regulating TET protein stability and exercise. These knowledge prolong our understanding of how TET proteins are regulated and supply new perception into the mechanisms of pVHL in tumor suppression.
Level-of-care DNA testing by routinely and sequentially performing extraction, amplification and identification in a closed-type cassette
- Nucleic acid detection is vital for scientific diagnostics; nonetheless, it’s difficult to carry out genetic testing on the point-of-care because of the tedious steps concerned in DNA extraction and the chance of cross-contamination from amplicons.
- To realize a fully-automated and contamination-free nucleic acid detection, we suggest a closed-type cassette system which allows the next steps to be operated routinely and sequentially: pattern preparation based mostly on magnetic beads, goal amplification utilizing multiplex polymerase chain response, and colorimetric detection of amplicons utilizing a serial invasive response coupled with the aggregation of gold nanoparticle probes.
- The cassette was designed to be spherical and closed, and 10 targets in a pattern might be concurrently detected by the bare eye or utilizing a spectrophotometer within the system.
- As well as, a cassette-driven system was fabricated to switch reagents between wells, to regulate the temperature of every response, and to sense the color within the detection wells. The cassette system was delicate sufficient to detect 10 genotypes at 5 single nucleotide polymorphism websites associated to the anticoagulant’s utilization, through the use of a 0.5 µL blood pattern.
- The accuracy of the system was evaluated by detecting 12 entire blood samples, and the outcomes obtained have been according to these obtained utilizing pyrosequencing. The cassette is hermetic and the entire system is absolutely computerized; the one guide operation is the addition of the pattern to the cassette, performing point-of-care genetic testing in a sample-in/answer-out means.
Recombinant Human E3 ubiquitin-protein ligase ZNRF3 (ZNRF3) ,partial |
||
| RPC23738-1mg | 1mg | EUR 2237.8 |
Recombinant Human E3 ubiquitin-protein ligase ZNRF3 (ZNRF3) ,partial |
||
| RPC23738-20ug | 20ug | EUR 258.7 |
Recombinant Human E3 ubiquitin-protein ligase ZNRF3 (ZNRF3) ,partial |
||
| RPC28709-100ug | 100ug | EUR 926.9 |
Recombinant Human E3 ubiquitin-protein ligase ZNRF3 (ZNRF3) ,partial |
||
| RPC28709-1mg | 1mg | EUR 2688 |
Recombinant Human E3 ubiquitin-protein ligase ZNRF3 (ZNRF3) ,partial |
||
| RPC28709-20ug | 20ug | EUR 366.1 |
Recombinant Human E3 ubiquitin-protein ligase ZNRF3 (ZNRF3) ,partial (Active) |
||
| RPC27883-100ug | 100ug | EUR 514.8 |
Recombinant Human E3 ubiquitin-protein ligase ZNRF3 (ZNRF3) ,partial (Active) |
||
| RPC27883-20ug | 20ug | EUR 309.5 |
Recombinant Human Probable E3 ubiquitin-protein ligase HERC1 (HERC1) ,partial |
||
| RPC27712-100ug | 100ug | EUR 801.9 |
Recombinant Human Probable E3 ubiquitin-protein ligase HERC1 (HERC1) ,partial |
||
| RPC27712-1mg | 1mg | EUR 2885.2 |
Recombinant Human Probable E3 ubiquitin-protein ligase HERC1 (HERC1) ,partial |
||
| RPC27712-20ug | 20ug | EUR 448.1 |
Recombinant Mouse Long-chain-fatty-acid--CoA ligase 4 (Acsl4) ,partial |
||
| RPC28393-100ug | 100ug | EUR 801.9 |
Recombinant Mouse Long-chain-fatty-acid--CoA ligase 4 (Acsl4) ,partial |
||
| RPC28393-1mg | 1mg | EUR 2885.2 |
Recombinant Mouse Long-chain-fatty-acid--CoA ligase 4 (Acsl4) ,partial |
||
| RPC28393-20ug | 20ug | EUR 448.1 |
Recombinant Arabidopsis thaliana E3 SUMO-protein ligase SIZ1 (SIZ1) ,partial |
||
| RPC27741-100ug | 100ug | EUR 801.9 |
Recombinant Arabidopsis thaliana E3 SUMO-protein ligase SIZ1 (SIZ1) ,partial |
||
| RPC27741-1mg | 1mg | EUR 2885.2 |
Recombinant Arabidopsis thaliana E3 SUMO-protein ligase SIZ1 (SIZ1) ,partial |
||
| RPC27741-20ug | 20ug | EUR 448.1 |
Recombinant Human Succinyl-CoA ligase [GDP-forming] subunit beta, mitochondrial (SUCLG2) ,partial |
||
| RPC23431-100ug | 100ug | EUR 460.9 |
Recombinant Human Succinyl-CoA ligase [GDP-forming] subunit beta, mitochondrial (SUCLG2) ,partial |
||
| RPC23431-1mg | 1mg | EUR 2237.8 |
Recombinant Human Succinyl-CoA ligase [GDP-forming] subunit beta, mitochondrial (SUCLG2) ,partial |
||
| RPC23431-20ug | 20ug | EUR 258.7 |
Human DNA Ligase 4 (LIG4) ELISA Kit |
||
| abx388272-96tests | 96 tests | EUR 1093.2 |
Human DNA ligase 4, LIG4 ELISA KIT |
||
| ELI-47684h | 96 Tests | EUR 988.8 |
DNA Ligase 4 (LIG4) Antibody |
||
| abx234774-100ug | 100 ug | EUR 610.8 |
DNA Ligase 4 (LIG4) Antibody |
||
| 20-abx141084 |
|
|
DNA Ligase 4 (LIG4) Antibody |
||
| 20-abx324859 |
|
|
DNA Ligase 4 (LIG4) Antibody |
||
| abx330937-100ul | 100 ul | EUR 510 |
DNA Ligase 4 (LIG4) Antibody |
||
| 20-abx113500 |
|
|
DNA Ligase 4 (LIG4) Antibody |
||
| abx034651-400ul | 400 ul | EUR 627.6 |
DNA Ligase 4 (LIG4) Antibody |
||
| abx034651-80l | 80 µl | EUR 343.2 |
DNA Ligase 4 (LIG4) Antibody |
||
| 20-abx322926 |
|
|
DNA Ligase 4 (LIG4) Antibody |
||
| 20-abx001451 |
|
|
DNA Ligase 4 (LIG4) Antibody |
||
| 20-abx009038 |
|
|
T4 DNA Ligase (Recombinant) |
||
| abx073011-100000IU | 100.000 IU | EUR 610.8 |
T4 DNA Ligase (Recombinant) |
||
| abx073011-20000IU | 20.000 IU | EUR 309.6 |
T4 DNA Ligase (Recombinant) |
||
| abx073011-500000IU | 500.000 IU | EUR 1846.8 |
Recombinant T4 DNA Ligase |
||
| 7-03622 | 20,000IU | Ask for price |
Recombinant T4 DNA Ligase |
||
| 7-03623 | 100,000IU | Ask for price |
Recombinant T4 DNA Ligase |
||
| 7-03624 | 500,000IU | Ask for price |
T4 DNA Ligase (Recombinant) |
||
| 22060706-1 | 20000 IU | EUR 146.42 |
Recombinant (E.Coli) T4 DNA Ligase |
||
| RP-374 | 20 KU | EUR 196.8 |
Recombinant ATP Dependent DNA ligase I (LIG1) |
||
| 4-RPB269Hu01 |
|
|
|
Description: Recombinant Human ATP Dependent DNA ligase I expressed in: E.coli |
||
Recombinant ATP Dependent DNA ligase I (LIG1) |
||
| 4-RPB269Ra01 |
|
|
|
Description: Recombinant Rat ATP Dependent DNA ligase I expressed in: E.coli |
||
Recombinant ATP Dependent DNA ligase I (LIG1) |
||
| RPU54179-100ug | 100ug | EUR 578.6 |
Recombinant ATP Dependent DNA ligase I (LIG1) |
||
| RPU54179-1mg | 1mg | EUR 2566.2 |
Recombinant ATP Dependent DNA ligase I (LIG1) |
||
| RPU54179-50ug | 50ug | EUR 465.3 |
Recombinant ATP Dependent DNA ligase I (LIG1) |
||
| RPU54517-100ug | 100ug | EUR 603.9 |
Recombinant ATP Dependent DNA ligase I (LIG1) |
||
| RPU54517-1mg | 1mg | EUR 2675.4 |
Recombinant ATP Dependent DNA ligase I (LIG1) |
||
| RPU54517-50ug | 50ug | EUR 485.1 |
Mouse DNA ligase 4, Lig4 ELISA KIT |
||
| ELI-32012m | 96 Tests | EUR 1038 |
Recombinant Human ICOS ligand (ICOSLG) ,partial (Active) |
||
| RPC28042-100ug | 100ug | EUR 449.4 |
Recombinant Human ICOS ligand (ICOSLG) ,partial (Active) |
||
| RPC28042-20ug | 20ug | EUR 273.6 |
Recombinant Human CD40 ligand (CD40LG) ,partial (Active) |
||
| RPC28049-100ug | 100ug | EUR 449.4 |
Recombinant Human CD40 ligand (CD40LG) ,partial (Active) |
||
| RPC28049-20ug | 20ug | EUR 273.6 |
Chicken DNA ligase 4, LIG4 ELISA KIT |
||
| ELI-47798c | 96 Tests | EUR 1113.6 |
Recombinant Human DNA ligase 1 Protein, GST, E.coli-1mg |
||
| QP6308-ec-1mg | 1mg | EUR 1958.4 |
Recombinant Human DNA ligase 1 Protein, GST, E.coli-10ug |
||
| QP6308-ec-10ug | 10ug | EUR 240 |
Recombinant Human DNA ligase 1 Protein, GST, E.coli-50ug |
||
| QP6308-ec-50ug | 50ug | EUR 315.6 |
Recombinant Human DNA ligase 1 Protein, GST, E.coli-100ug |
||
| QP6308-ec-100ug | 100ug | EUR 489.6 |
Recombinant Human DNA ligase 1 Protein, GST, E.coli-200ug |
||
| QP6308-ec-200ug | 200ug | EUR 760.8 |
Recombinant Human DNA ligase 1 Protein, GST, E.coli-500ug |
||
| QP6308-ec-500ug | 500ug | EUR 1272 |
Recombinant Human DNA polymerase theta (POLQ) ,partial |
||
| RPC27274-100ug | 100ug | EUR 635.8 |
Recombinant Human DNA polymerase theta (POLQ) ,partial |
||
| RPC27274-1mg | 1mg | EUR 2885.2 |
Recombinant Human DNA polymerase theta (POLQ) ,partial |
||
| RPC27274-20ug | 20ug | EUR 353.4 |
DNA Ligase IV recombinant monoclonal antibody |
||
| A5820 | 100ul X 3 | EUR 714 |
|
Description: A recombinant monoclonal antibody from rabbit against human DNA Ligase IV for WB, IHC, IF,ELISA |
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Recombinant Rabbit CD40 ligand (CD40LG) ,partial |
||
| RPC24569-100ug | 100ug | EUR 1007.6 |
Recombinant Rabbit CD40 ligand (CD40LG) ,partial |
||
| RPC24569-20ug | 20ug | EUR 479.6 |
Recombinant Rabbit CD40 ligand (CD40LG) ,partial |
||
| RPC21056-100ug | 100ug | EUR 721.6 |
Recombinant Rabbit CD40 ligand (CD40LG) ,partial |
||
| RPC21056-1mg | 1mg | EUR 2884.7 |
Recombinant Rabbit CD40 ligand (CD40LG) ,partial |
||
| RPC21056-20ug | 20ug | EUR 448.1 |
Recombinant Human Programmed cell death 1 ligand 1 (CD274) ,partial |
||
| RPC27799-100ug | 100ug | EUR 460.9 |
Recombinant Human Programmed cell death 1 ligand 1 (CD274) ,partial |
||
| RPC27799-1mg | 1mg | EUR 2237.8 |
Recombinant Human Programmed cell death 1 ligand 1 (CD274) ,partial |
||
| RPC27799-20ug | 20ug | EUR 258.7 |
Recombinant Human Netrin-4 (NTN4) ,partial |
||
| RPC28746-100ug | 100ug | EUR 635.8 |
Recombinant Human Netrin-4 (NTN4) ,partial |
||
| RPC28746-1mg | 1mg | EUR 2885.2 |
Recombinant Human Netrin-4 (NTN4) ,partial |
||
| RPC28746-20ug | 20ug | EUR 353.4 |
Recombinant Human Programmed cell death 1 ligand 1 (CD274) ,partial (Active) |
||
| RPC26433-100ug | 100ug | EUR 449.4 |
Recombinant Human Programmed cell death 1 ligand 1 (CD274) ,partial (Active) |
||
| RPC26433-20ug | 20ug | EUR 273.6 |
Recombinant E.coli DNA ligase Protein, His, E.coli-1mg |
||
| QP7075-1mg | 1mg | EUR 3037.2 |
DNA Ligase 4 Phospho-Thr650 (LIG4 pT650) Antibody |
||
| 20-abx322874 |
|
|
Recombinant Human Aquaporin-4 (AQP4) ,partial |
||
| RPC20006-100ug | 100ug | EUR 460.9 |
Recombinant Human Aquaporin-4 (AQP4) ,partial |
||
| RPC20006-1mg | 1mg | EUR 2237.8 |
Recombinant Human Aquaporin-4 (AQP4) ,partial |
||
| RPC20006-20ug | 20ug | EUR 258.7 |
Recombinant E.coli DNA ligase Protein, His, E.coli-10ug |
||
| QP7075-10ug | 10ug | EUR 426 |
Recombinant E.coli DNA ligase Protein, His, E.coli-50ug |
||
| QP7075-50ug | 50ug | EUR 567.6 |
Recombinant Human TAR DNA-binding protein 43 (TARDBP) ,partial |
||
| RPC24976-100ug | 100ug | EUR 573.1 |
Recombinant Human TAR DNA-binding protein 43 (TARDBP) ,partial |
||
| RPC24976-20ug | 20ug | EUR 288.8 |
Recombinant Macaca mulatta CD40 ligand (CD40LG) ,partial |
||
| RPC21055-100ug | 100ug | EUR 721.6 |
Recombinant Macaca mulatta CD40 ligand (CD40LG) ,partial |
||
| RPC21055-1mg | 1mg | EUR 2884.7 |
Recombinant Macaca mulatta CD40 ligand (CD40LG) ,partial |
||
| RPC21055-20ug | 20ug | EUR 448.1 |
Recombinant E.coli DNA ligase Protein, His, E.coli-100ug |
||
| QP7075-100ug | 100ug | EUR 925.2 |
Recombinant E.coli DNA ligase Protein, His, E.coli-200ug |
||
| QP7075-200ug | 200ug | EUR 1468.8 |