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Convertex Chemie GmbH – Herstellung von Spezialchemikalien

Convertex Chemie GmbH – Herstellung von Spezialchemikalien

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MCDB 131 W/O L-Glutamin

Author Hans Posted on Juni 3, 2022Juni 4, 2022Categories Assay Kits, RNATags rna base pairs, rna bases, rna definition, rna extraction, rna interference, rna medical, rna polymerase, rna processing, rna reset, rna sequencing, rna structure, rna transcription, rna vaccine, rna virus, rna vs dna, rna-seq, rnafold, rnai, rnalater, rnascope, rnase, rnaseq, rnation login, rnav   Leave a comment on MCDB 131 W/O L-Glutamin

Protocols for maintenance of hPSCs and generation of lung cells were slightly modified from previous studies (Chen et al., 2019; Huang et al., 2014). The hESC line-RUES2 was cultured on irradiated mouse embryonic fibroblasts (Global Stem, cat. no. GSC-6001G) at a density of 20,000-25,000 cells/cm2 in a medium of DMEM/F12, 20% knockout serum replacement (Life Technologies ), 0.1 mM β-mercaptoethanol (Sigma Aldrich) and 20 ng/ml bFGF (R&D Systems), and medium was changed daily. hESC cultures were maintained in an undifferentiated … Continue reading „MCDB 131 W/O L-Glutamin“

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