Protocols for maintenance of hPSCs and generation of lung cells were slightly modified from previous studies (Chen et al., 2019; Huang et al., 2014). The hESC line-RUES2 was cultured on irradiated mouse embryonic fibroblasts (Global Stem, cat. no. GSC-6001G) at a density of 20,000-25,000 cells/cm2 in a medium of DMEM/F12, 20% knockout serum replacement (Life Technologies ), 0.1 mM β-mercaptoethanol (Sigma Aldrich) and 20 ng/ml bFGF (R&D Systems), and medium was changed daily. hESC cultures were maintained in an undifferentiated state at 37 °C in a 5% CO2/air environment until stem cells reached about 90% confluence.
Pancreatic endocrine cell differentiation was performed using INSGFP/W MEL-1 cells.
Cells were cultured on Matrigel-coated 6-well plates in StemFlex medium (Gibco Thermo Fisher). Cells were maintained at 37℃ with 5% CO2. hESCs were differentiated using a previously reported strategy (Zeng et al., 2016). In brief, on day 0, cells were exposed to basal medium RPMI 1640 supplemented with 1× Glutamax (Thermo Fisher Scientific), 50 μg/mL Normocin, 100 ng/mL Activin A (R&D systems), and 2 μM of CHIR99021 (GSK3β inhibitor 3, Cayman Chemical ) for 24 h. The medium was changed on day 1 to basal RPMI 1640 medium supplemented with 1× Glutamax (Thermo Fisher Scientific), 50 μg/mL normocin, 0.2% fetal bovine serum (FBS, Corning), 100 ng/mLActivin A (R&D systems) for 2 days.
On day 3, the resulting definitive endoderm cells were cultured in MCDB 131 medium (Thermo Fisher Scientific) supplemented with 1.5 g/L sodium bicarbonate, 1× Glutamax, 10 mM glucose (Sigma Aldrich) at final concentration, 2% bovine serum albumin ( BSA, Lampire), 0.25 mM L-ascorbic acid (Sigma Aldrich) and 50 ng/ml of fibroblast growth factor 7 (FGF-7, Peprotech) for 2 days to acquire primitive gut tube. On day 5, the cells were induced to differentiate to posterior foregut in MCDB 131 medium supplemented with 2.5 g/L sodium bicarbonate, 1× Glutamax, 10 mM glucose at final concentration, 2% BSA, 0.25 mM L-ascorbic acid, 50 ng /ml of FGF-7, 1 μM Retinoic acid (RA; Sigma Aldrich), 100 nM LDN193189 (LDN, Axon Medchem), 1:200 ITS-X (Thermo Fisher Scientific), 200 nM TPPB (Tocris Bioscience) and 0.
On day 7, the cells were induced to differentiate to pancreatic endoderm in MCDB 131 medium supplemented with 2.5 g/L sodium bicarbonate, 1× Glutamax, 10 mM glucose at final concentration, 2% BSA, 0.25 mM L-ascorbic acid, 2 ng /ml of FGF-7, 0.1 μM RA, 200 nM LDN193189, 1:200 ITS-X, 100 nM TPPB and 0.25 μM SANT-1 for 3 days.On day 10, the cells were induced to differentiate to pancreatic endocrine precursors in MCDB 131 medium supplemented with 1.5 g/L sodium bicarbonate, 1× Glutamax, 20 mM glucose at final concentration, 2% BSA, 0.05 μM RA, 100 nM LDN, 1:200 ITS-X, 0.25 μM SANT-1, 1 mM T3 hormone (Sigma Aldrich), 10 μM ALK5 inhibitor II (Cayman Chemical), 10 μM zinc sulfate heptahydrate (Sigma Aldrich) and 10 μg/ml of heparin (Sigma Aldrich) for 3 days.
On day 13, cells were exposed to MCDB 131 medium supplemented with 1.5 g/L sodium bicarbonate, 1× Glutamax, 20 mM glucose at final concentration, 2% BSA, 100 nM LDN193189, 1:200 ITS-X, 1 μM T3, 10 μM ALK5 inhibitor II, 10 μM zinc sulfate, 10 μg/ml of heparin, 100 nM gamma secretase inhibitor XX (Millipore) for the first 7 days. On day 27, cells were exposed to MCDB 131 medium supplemented with 1. 5 g/L sodium bicarbonate, 1× Glutamax, 20 mM glucose at final concentration, 2% BSA, 1:200 ITS-X, 1 μM T3, 10 μM ALK5 inhibitor II, 10 μM zinc sulfate heptahydrate, 10 μg/ml of heparin, 1 mM N-acetyl cysteine (Sigma Aldrich), 10 μM Trolox (Millipore), 2 μM R428 (MedchemExpress) for 7-15 days. The medium was subsequently refreshed every day.
Organoid infections were performed at an MOI of 0.1 and harvested at 24 hpi in DMEM supplemented with 0.3% BSA, 4.5 g/L D-glucose, 4 mM L-glutamine and 1 μg/ml TPCKtrypsin.
Total RNA was extracted in TRIzol (Invitrogen) and DNase I treated using Directzol RNA Miniprep kit (Zymo Research) according to the manufacturer’s instructions. cDNA libraries were sequenced using an Illumina NextSeq 500 platform. The resulting single end reads were checked for quality (FastQC v0.11.5) and processed using the Digital Expression Explorer 2 (DEE2) (Ziemann et al., 2019) workflow. Adapter trimming was performed with Skewer (v0.2.2) (Jiang et al., 2014).
Rat glutamine enzyme (glutaminase) ELISA Kit
YLA1458RA-48T
Shanghai YL Biotech
48T
Ask for price
Rat glutamine enzyme (glutaminase) ELISA Kit
YLA1458RA-96T
Shanghai YL Biotech
96T
Ask for price
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase) (FITC)
MBS6149153-01mL
MyBiosource
0.1(mL
875 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase) (FITC)
MBS6149153-5x01mL
MyBiosource
5x0.1mL
3800 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase) (HRP)
MBS6154456-01mL
MyBiosource
0.1(mL
875 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase) (HRP)
MBS6154456-5x01mL
MyBiosource
5x0.1mL
3800 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase) (PE)
MBS6159759-01mL
MyBiosource
0.1(mL
875 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase) (PE)
MBS6159759-5x01mL
MyBiosource
5x0.1mL
3800 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase)
MBS6003746-01mg
MyBiosource
0.1(mg
630 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase)
MBS6003746-5x01mg
MyBiosource
5x0.1mg
2680 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase) APC
MBS6138547-01mL
MyBiosource
0.1(mL
875 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase) APC
MBS6138547-5x01mL
MyBiosource
5x0.1mL
3800 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase) (AP)
MBS6133244-01mL
MyBiosource
0.1(mL
875 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase) (AP)
MBS6133244-5x01mL
MyBiosource
5x0.1mL
3800 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase) (AP)
MBS6391638-01mL
MyBiosource
0.1mL
920 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase) (AP)
MBS6391638-5x01mL
MyBiosource
5x0.1mL
3990 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase) APC
MBS6391639-01mL
MyBiosource
0.1mL
920 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase) APC
MBS6391639-5x01mL
MyBiosource
5x0.1mL
3990 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase) (FITC)
MBS6391641-01mL
MyBiosource
0.1mL
920 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase) (FITC)
MBS6391641-5x01mL
MyBiosource
5x0.1mL
3990 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase) (HRP)
MBS6391642-01mL
MyBiosource
0.1mL
920 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase) (HRP)
MBS6391642-5x01mL
MyBiosource
5x0.1mL
3990 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase) (PE)
MBS6391648-01mL
MyBiosource
0.1mL
920 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase) (PE)
MBS6391648-5x01mL
MyBiosource
5x0.1mL
3990 EUR
QPCT, CT (QPCT, Glutaminyl-peptide cyclotransferase, Glutaminyl cyclase, Glutaminyl-tRNA cyclotransferase, Glutamyl cyclase) (AP)
MBS6339415-02mL
MyBiosource
0.2mL
980 EUR
QPCT, CT (QPCT, Glutaminyl-peptide cyclotransferase, Glutaminyl cyclase, Glutaminyl-tRNA cyclotransferase, Glutamyl cyclase) (AP)
MBS6339415-5x02mL
MyBiosource
5x0.2mL
4250 EUR
QPCT, CT (QPCT, Glutaminyl-peptide cyclotransferase, Glutaminyl cyclase, Glutaminyl-tRNA cyclotransferase, Glutamyl cyclase) (PE)
MBS6339425-02mL
MyBiosource
0.2mL
980 EUR
QPCT, CT (QPCT, Glutaminyl-peptide cyclotransferase, Glutaminyl cyclase, Glutaminyl-tRNA cyclotransferase, Glutamyl cyclase) (PE)
MBS6339425-5x02mL
MyBiosource
5x0.2mL
4250 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase, QC, GCT, sQC) (AP)
MBS6448824-01mL
MyBiosource
0.1mL
950 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase, QC, GCT, sQC) (AP)
MBS6448824-5x01mL
MyBiosource
5x0.1mL
4120 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase, QC, GCT, sQC) (PE)
MBS6448834-01mL
MyBiosource
0.1mL
950 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase, QC, GCT, sQC) (PE)
MBS6448834-5x01mL
MyBiosource
5x0.1mL
4120 EUR
QPCT, CT (QPCT, Glutaminyl-peptide cyclotransferase, Glutaminyl cyclase, Glutaminyl-tRNA cyclotransferase, Glutamyl cyclase)
MBS641484-02mL
MyBiosource
0.2mL
695 EUR
QPCT, CT (QPCT, Glutaminyl-peptide cyclotransferase, Glutaminyl cyclase, Glutaminyl-tRNA cyclotransferase, Glutamyl cyclase)
MBS641484-5x02mL
MyBiosource
5x0.2mL
2975 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase)
MBS642109-01mg
MyBiosource
0.1mg
665 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase)
MBS642109-5x01mg
MyBiosource
5x0.1mg
2850 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase)
MBS648239-005mg
MyBiosource
0.05mg
630 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase)
MBS648239-5x005mg
MyBiosource
5x0.05mg
2680 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase) (MaxLight 490)
MBS6202783-01mL
MyBiosource
0.1(mL
875 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase) (MaxLight 490)
MBS6202783-5x01mL
MyBiosource
5x0.1mL
3800 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase) (MaxLight 550)
MBS6213458-01mL
MyBiosource
0.1(mL
875 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase) (MaxLight 550)
MBS6213458-5x01mL
MyBiosource
5x0.1mL
3800 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase) (MaxLight 405)
MBS6192108-01mL
MyBiosource
0.1(mL
875 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase) (MaxLight 405)
MBS6192108-5x01mL
MyBiosource
5x0.1mL
3800 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase) (MaxLight 750)
MBS6234808-01mL
MyBiosource
0.1(mL
875 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase) (MaxLight 750)
MBS6234808-5x01mL
MyBiosource
5x0.1mL
3800 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase) (MaxLight 650)
MBS6224133-01mL
MyBiosource
0.1(mL
875 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase) (MaxLight 650)
MBS6224133-5x01mL
MyBiosource
5x0.1mL
3800 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase) (Biotin)
MBS6143850-01mL
MyBiosource
0.1(mL
875 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase) (Biotin)
MBS6143850-5x01mL
MyBiosource
5x0.1mL
3800 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase) (Biotin)
MBS6391640-01mL
MyBiosource
0.1mL
920 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase) (Biotin)
MBS6391640-5x01mL
MyBiosource
5x0.1mL
3990 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase) (MaxLight 405)
MBS6391643-01mL
MyBiosource
0.1mL
920 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase) (MaxLight 405)
MBS6391643-5x01mL
MyBiosource
5x0.1mL
3990 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase) (MaxLight 490)
MBS6391644-01mL
MyBiosource
0.1mL
920 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase) (MaxLight 490)
MBS6391644-5x01mL
MyBiosource
5x0.1mL
3990 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase) (MaxLight 550)
MBS6391645-01mL
MyBiosource
0.1mL
920 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase) (MaxLight 550)
MBS6391645-5x01mL
MyBiosource
5x0.1mL
3990 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase) (MaxLight 650)
MBS6391646-01mL
MyBiosource
0.1mL
920 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase) (MaxLight 650)
MBS6391646-5x01mL
MyBiosource
5x0.1mL
3990 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase) (MaxLight 750)
MBS6391647-01mL
MyBiosource
0.1mL
920 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase) (MaxLight 750)
MBS6391647-5x01mL
MyBiosource
5x0.1mL
3990 EUR
QPCT, CT (QPCT, Glutaminyl-peptide cyclotransferase, Glutaminyl cyclase, Glutaminyl-tRNA cyclotransferase, Glutamyl cyclase) (APC)
MBS6339416-02mL
MyBiosource
0.2mL
980 EUR
QPCT, CT (QPCT, Glutaminyl-peptide cyclotransferase, Glutaminyl cyclase, Glutaminyl-tRNA cyclotransferase, Glutamyl cyclase) (APC)
MBS6339416-5x02mL
MyBiosource
5x0.2mL
4250 EUR
QPCT, CT (QPCT, Glutaminyl-peptide cyclotransferase, Glutaminyl cyclase, Glutaminyl-tRNA cyclotransferase, Glutamyl cyclase) (Biotin)
MBS6339417-02mL
MyBiosource
0.2mL
980 EUR
QPCT, CT (QPCT, Glutaminyl-peptide cyclotransferase, Glutaminyl cyclase, Glutaminyl-tRNA cyclotransferase, Glutamyl cyclase) (Biotin)
MBS6339417-5x02mL
MyBiosource
5x0.2mL
4250 EUR
QPCT, CT (QPCT, Glutaminyl-peptide cyclotransferase, Glutaminyl cyclase, Glutaminyl-tRNA cyclotransferase, Glutamyl cyclase) (FITC)
MBS6339418-02mL
MyBiosource
0.2mL
980 EUR
QPCT, CT (QPCT, Glutaminyl-peptide cyclotransferase, Glutaminyl cyclase, Glutaminyl-tRNA cyclotransferase, Glutamyl cyclase) (FITC)
MBS6339418-5x02mL
MyBiosource
5x0.2mL
4250 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase, QC, GCT, sQC) (APC)
MBS6448825-01mL
MyBiosource
0.1mL
950 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase, QC, GCT, sQC) (APC)
MBS6448825-5x01mL
MyBiosource
5x0.1mL
4120 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase, QC, GCT, sQC) (Biotin)
MBS6448826-01mL
MyBiosource
0.1mL
950 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase, QC, GCT, sQC) (Biotin)
MBS6448826-5x01mL
MyBiosource
5x0.1mL
4120 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase, QC, GCT, sQC) (FITC)
MBS6448827-01mL
MyBiosource
0.1mL
950 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase, QC, GCT, sQC) (FITC)
MBS6448827-5x01mL
MyBiosource
5x0.1mL
4120 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase, QC, GCT, sQC) (HRP).
MBS6448828-01mL
MyBiosource
0.1mL
950 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase, QC, GCT, sQC) (HRP).
MBS6448828-5x01mL
MyBiosource
5x0.1mL
4120 EUR
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS) (HRP)
MBS6154454-01mL
MyBiosource
0.1(mL
875 EUR
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS) (HRP)
MBS6154454-5x01mL
MyBiosource
5x0.1mL
3800 EUR
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS) (PE)
MBS6159757-01mL
MyBiosource
0.1(mL
875 EUR
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS) (PE)
MBS6159757-5x01mL
MyBiosource
5x0.1mL
3800 EUR
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS) APC
MBS6138545-01mL
MyBiosource
0.1(mL
875 EUR
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS) APC
MBS6138545-5x01mL
MyBiosource
5x0.1mL
3800 EUR
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS) (AP)
MBS6133242-01mL
MyBiosource
0.1(mL
875 EUR
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS) (AP)
MBS6133242-5x01mL
MyBiosource
5x0.1mL
3800 EUR
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS) (AP)
MBS6391627-01mL
MyBiosource
0.1mL
920 EUR
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS) (AP)
MBS6391627-5x01mL
MyBiosource
5x0.1mL
3990 EUR
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS) APC
MBS6391628-01mL
MyBiosource
0.1mL
920 EUR
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS) APC
MBS6391628-5x01mL
MyBiosource
5x0.1mL
3990 EUR
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS) (HRP)
MBS6391631-01mL
MyBiosource
0.1mL
920 EUR
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS) (HRP)
MBS6391631-5x01mL
MyBiosource
5x0.1mL
3990 EUR
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS) (PE)
MBS6391637-01mL
MyBiosource
0.1mL
920 EUR
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS) (PE)
MBS6391637-5x01mL
MyBiosource
5x0.1mL
3990 EUR
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS)
MBS6012851-01mg
MyBiosource
0.1(mg
630 EUR
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS)
MBS6012851-5x01mg
MyBiosource
5x0.1mg
2680 EUR
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS)
MBS6011922-01mg
MyBiosource
0.1(mg
665 EUR
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS)
MBS6011922-5x01mg
MyBiosource
5x0.1mg
2850 EUR
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS)
MBS6012285-005mg
MyBiosource
0.05(mg
630 EUR
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS)
MBS6012285-5x005mg
MyBiosource
5x0.05mg
2680 EUR
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS) (FITC)
MBS6149151-01mL
MyBiosource
0.1(mL
875 EUR
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS) (FITC)
MBS6149151-5x01mL
MyBiosource
5x0.1mL
3800 EUR
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS) (FITC)
MBS6391630-01mL
MyBiosource
0.1mL
920 EUR
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS) (FITC)
MBS6391630-5x01mL
MyBiosource
5x0.1mL
3990 EUR
QPCT, CT (QPCT, Glutaminyl-peptide cyclotransferase, Glutaminyl cyclase, Glutaminyl-tRNA cyclotransferase, Glutamyl cyclase) (Azide free) (HRP)
MBS6339419-02mL
MyBiosource
0.2mL
980 EUR
QPCT, CT (QPCT, Glutaminyl-peptide cyclotransferase, Glutaminyl cyclase, Glutaminyl-tRNA cyclotransferase, Glutamyl cyclase) (Azide free) (HRP)
MBS6339419-5x02mL
MyBiosource
5x0.2mL
4250 EUR
QPCT, CT (QPCT, Glutaminyl-peptide cyclotransferase, Glutaminyl cyclase, Glutaminyl-tRNA cyclotransferase, Glutamyl cyclase) (MaxLight 405)
MBS6339420-01mL
MyBiosource
0.1mL
980 EUR
QPCT, CT (QPCT, Glutaminyl-peptide cyclotransferase, Glutaminyl cyclase, Glutaminyl-tRNA cyclotransferase, Glutamyl cyclase) (MaxLight 405)
MBS6339420-5x01mL
MyBiosource
5x0.1mL
4250 EUR
QPCT, CT (QPCT, Glutaminyl-peptide cyclotransferase, Glutaminyl cyclase, Glutaminyl-tRNA cyclotransferase, Glutamyl cyclase) (MaxLight 490)
MBS6339421-01mL
MyBiosource
0.1mL
980 EUR
QPCT, CT (QPCT, Glutaminyl-peptide cyclotransferase, Glutaminyl cyclase, Glutaminyl-tRNA cyclotransferase, Glutamyl cyclase) (MaxLight 490)
MBS6339421-5x01mL
MyBiosource
5x0.1mL
4250 EUR
QPCT, CT (QPCT, Glutaminyl-peptide cyclotransferase, Glutaminyl cyclase, Glutaminyl-tRNA cyclotransferase, Glutamyl cyclase) (MaxLight 550)
MBS6339422-01mL
MyBiosource
0.1mL
980 EUR
QPCT, CT (QPCT, Glutaminyl-peptide cyclotransferase, Glutaminyl cyclase, Glutaminyl-tRNA cyclotransferase, Glutamyl cyclase) (MaxLight 550)
MBS6339422-5x01mL
MyBiosource
5x0.1mL
4250 EUR
QPCT, CT (QPCT, Glutaminyl-peptide cyclotransferase, Glutaminyl cyclase, Glutaminyl-tRNA cyclotransferase, Glutamyl cyclase) (MaxLight 650)
MBS6339423-01mL
MyBiosource
0.1mL
980 EUR
QPCT, CT (QPCT, Glutaminyl-peptide cyclotransferase, Glutaminyl cyclase, Glutaminyl-tRNA cyclotransferase, Glutamyl cyclase) (MaxLight 650)
MBS6339423-5x01mL
MyBiosource
5x0.1mL
4250 EUR
QPCT, CT (QPCT, Glutaminyl-peptide cyclotransferase, Glutaminyl cyclase, Glutaminyl-tRNA cyclotransferase, Glutamyl cyclase) (MaxLight 750)
MBS6339424-01mL
MyBiosource
0.1mL
980 EUR
QPCT, CT (QPCT, Glutaminyl-peptide cyclotransferase, Glutaminyl cyclase, Glutaminyl-tRNA cyclotransferase, Glutamyl cyclase) (MaxLight 750)
MBS6339424-5x01mL
MyBiosource
5x0.1mL
4250 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase, QC, GCT, sQC) (MaxLight 405)
MBS6448829-01mL
MyBiosource
0.1mL
950 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase, QC, GCT, sQC) (MaxLight 405)
MBS6448829-5x01mL
MyBiosource
5x0.1mL
4120 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase, QC, GCT, sQC) (MaxLight 490)
MBS6448830-01mL
MyBiosource
0.1mL
950 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase, QC, GCT, sQC) (MaxLight 490)
MBS6448830-5x01mL
MyBiosource
5x0.1mL
4120 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase, QC, GCT, sQC) (MaxLight 550)
MBS6448831-01mL
MyBiosource
0.1mL
950 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase, QC, GCT, sQC) (MaxLight 550)
MBS6448831-5x01mL
MyBiosource
5x0.1mL
4120 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase, QC, GCT, sQC) (MaxLight 650)
MBS6448832-01mL
MyBiosource
0.1mL
950 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase, QC, GCT, sQC) (MaxLight 650)
MBS6448832-5x01mL
MyBiosource
5x0.1mL
4120 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase, QC, GCT, sQC) (MaxLight 750)
MBS6448833-01mL
MyBiosource
0.1mL
950 EUR
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase, QC, GCT, sQC) (MaxLight 750)
MBS6448833-5x01mL
MyBiosource
5x0.1mL
4120 EUR
Glutamine
596425
MedKoo Biosciences
250.0g
330 EUR
L-(+)-Glutamine
G02500
Pfaltz & Bauer
25G
111.3 EUR
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS) (Biotin)
MBS6143848-01mL
MyBiosource
0.1(mL
875 EUR
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS) (Biotin)
MBS6143848-5x01mL
MyBiosource
5x0.1mL
3800 EUR
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS) (Biotin)
MBS6391629-01mL
MyBiosource
0.1mL
920 EUR
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS) (Biotin)
MBS6391629-5x01mL
MyBiosource
5x0.1mL
3990 EUR
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS) (MaxLight 490)
MBS6202781-01mL
MyBiosource
0.1(mL
875 EUR
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS) (MaxLight 490)
MBS6202781-5x01mL
MyBiosource
5x0.1mL
3800 EUR
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS) (MaxLight 550)
MBS6213456-01mL
MyBiosource
0.1(mL
875 EUR
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS) (MaxLight 550)
MBS6213456-5x01mL
MyBiosource
5x0.1mL
3800 EUR
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS) (MaxLight 405)
MBS6192106-01mL
MyBiosource
0.1(mL
875 EUR
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS) (MaxLight 405)
MBS6192106-5x01mL
MyBiosource
5x0.1mL
3800 EUR
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS) (MaxLight 750)
MBS6234806-01mL
MyBiosource
0.1(mL
875 EUR
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS) (MaxLight 750)
MBS6234806-5x01mL
MyBiosource
5x0.1mL
3800 EUR
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS) (MaxLight 650)
MBS6224131-01mL
MyBiosource
0.1(mL
875 EUR
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS) (MaxLight 650)
MBS6224131-5x01mL
MyBiosource
5x0.1mL
3800 EUR
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS) (MaxLight 405)
MBS6391632-01mL
MyBiosource
0.1mL
920 EUR
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS) (MaxLight 405)
MBS6391632-5x01mL
MyBiosource
5x0.1mL
3990 EUR
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS) (MaxLight 490)
MBS6391633-01mL
MyBiosource
0.1mL
920 EUR
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS) (MaxLight 490)
MBS6391633-5x01mL
MyBiosource
5x0.1mL
3990 EUR
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS) (MaxLight 550)
MBS6391634-01mL
MyBiosource
0.1mL
920 EUR
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS) (MaxLight 550)
MBS6391634-5x01mL
MyBiosource
5x0.1mL
3990 EUR
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS) (MaxLight 650)
MBS6391635-01mL
MyBiosource
0.1mL
920 EUR
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS) (MaxLight 650)
MBS6391635-5x01mL
MyBiosource
5x0.1mL
3990 EUR
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS) (MaxLight 750)
MBS6391636-01mL
MyBiosource
0.1mL
920 EUR
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS) (MaxLight 750)
MBS6391636-5x01mL
MyBiosource
5x0.1mL
3990 EUR
Glutamine (Gln) (OVA)
abx651914-100g
Abbexa
100 µg
1962.5 EUR
Glutamine (Gln) (OVA)
abx651914-10g
Abbexa
10 µg
512.5 EUR
Glutamine (Gln) (OVA)
abx651914-50g
Abbexa
50 µg
612.5 EUR
Glutaminase
E8ET1611-5
EnoGene
100ul
275 EUR
Glutaminase
MBS8534061-01mL
MyBiosource
0.1mL
345 EUR
Glutaminase
MBS8534061-01mLAF405L
MyBiosource
0.1mL(AF405L)
565 EUR
Glutaminase
MBS8534061-01mLAF405S
MyBiosource
0.1mL(AF405S)
565 EUR
Glutaminase
MBS8534061-01mLAF610
MyBiosource
0.1mL(AF610)
565 EUR
Glutaminase
MBS8534061-01mLAF635
MyBiosource
0.1mL(AF635)
565 EUR
Glutaminase C-IN-1 (968)
B1198-25
Biovision
each
757.2 EUR
Glutaminase C-IN-1 (968)
B1198-5
Biovision
each
235.2 EUR
Stable Glutamine (200 mM) - 100 ml
CASTA-B
Westburg
each
23.54 EUR
Glutaminase C-IN-1
HY-12682
MedChemExpress
10mM/1mL
378.79 EUR
Glutamine ELISA Kit
ECP7865
Genovis AB
96 Tests
634 EUR
Glutaminase C-IN-2
HY-149602
MedChemExpress
Get quote
Ask for price
Glutamine ELISA kit
IS-I-1100R
ImmuSmol SAS
1 kit
523.8 EUR
Glutaminase C-IN-1
MBS579879-100mg
MyBiosource
100mg
445 EUR
Glutaminase C-IN-1
MBS579879-10mg
MyBiosource
10mg
180 EUR
Glutaminase C-IN-1
MBS579879-25mg
MyBiosource
25mg
250 EUR
Glutaminase C-IN-1
MBS579879-50mg
MyBiosource
50mg
345 EUR
Glutaminase C-IN-1
MBS579879-5mg
MyBiosource
5mg
155 EUR
Glutamine ELISA Kit
MBS288136-10x96StripWells
MyBiosource
10x96-Strip-Wells
4630 EUR
Glutamine ELISA Kit
MBS288136-48StripWells
MyBiosource
48-Strip-Wells
440 EUR
Glutamine ELISA Kit
MBS288136-5x96StripWells
MyBiosource
5x96-Strip-Wells
2480 EUR
Glutamine ELISA Kit
MBS288136-96StripWells
MyBiosource
96-Strip-Wells
580 EUR
Glutaminase C-IN-1
MBS3607467-10mg
MyBiosource
10mg
270 EUR
Glutaminase C-IN-1
MBS3607467-25mg
MyBiosource
25mg
385 EUR
Glutaminase C-IN-1
MBS3607467-2mg
MyBiosource
2mg
200 EUR
Glutaminase C-IN-1
MBS3607467-50mg
MyBiosource
50mg
605 EUR
Glutaminase C-IN-1
MBS3607467-5mg
MyBiosource
5mg
225 EUR
Glutaminase C-IN-1
T3964-10mg
TargetMol Chemicals
10mg
Ask for price
Glutaminase C-IN-1
T3964-1g
TargetMol Chemicals
1g
Ask for price
Glutaminase C-IN-1
T3964-1mg
TargetMol Chemicals
1mg
Ask for price
Glutaminase C-IN-1
T3964-50mg
TargetMol Chemicals
50mg
Ask for price
Glutaminase C-IN-1
T3964-5mg
TargetMol Chemicals
5mg
Ask for price
QRSL1 (untagged)-Human glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 (QRSL1)
SC320296
Origene Technologies GmbH
10 µg
Ask for price
QRSL1 (untagged)-Human glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 (QRSL1)
SC113600
Origene Technologies GmbH
10 µg
Ask for price
Qrsl1 (untagged ORF) - Rat glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 (Qrsl1), (10 ug)
RN211743
Origene Technologies GmbH
10 µg
Ask for price
Rat Glutamine (Glu) ELISA Kit
MBS2601106-10x96StripWells
MyBiosource
10x96-Strip-Wells
3290 EUR
Rat Glutamine (Glu) ELISA Kit
MBS2601106-48StripWells
MyBiosource
48-Strip-Wells
295 EUR
Rat Glutamine (Glu) ELISA Kit
MBS2601106-5x96StripWells
MyBiosource
5x96-Strip-Wells
1800 EUR
Rat Glutamine (Glu) ELISA Kit
MBS2601106-96StripWells
MyBiosource
96-Strip-Wells
450 EUR
Glutamine (Gln) Assay Kit
abx298914-100g
Abbexa
100 µg
Ask for price
Glutamine (Gln) Assay Kit
abx298914-20g
Abbexa
20 µg
475 EUR
Glutamine (Gln) Assay Kit
abx298914-50g
Abbexa
50 µg
Ask for price
Glutamine (Gln) ELISA Kit
abx585664-100g
Abbexa
100 µg
6187.5 EUR
Glutamine (Gln) ELISA Kit
abx585664-10g
Abbexa
10 µg
687.5 EUR
Glutamine (Gln) ELISA Kit
abx585664-50g
Abbexa
50 µg
3275 EUR
Glutamine (GLN) ELISA Kit
abx519584-96tests
Abbexa
96 tests
801.6 EUR
Further quality control done with Minion, part of the Kraken package (Davis et al., 2013). The resulting filtered reads were mapped to human reference genome GRCh38 using STAR aligner (Dobin et al., 2013) and gene-wise expression counts generated using the “-quantMode GeneCounts” parameter. BigWig files were generated using the bamCoverage function in deepTools2 (v.3.3.0) (Ramirez et al., 2016).
RNAseq libraries of polyadenylated RNA were prepared using the TruSeq RNA Library Prep Kit v2 (Illumina) or TruSeq Stranded mRNA Library Prep Kit (Illumina) according to the manufacturer’s instructions.